In vitro fertilization has served as a great tool in the human and bovine field in its ability to treat infertility in females. The purpose of this study was to compare hormone injection treatments in goats using the laparoscopic ovum pickup technique. A crossover trial was conducted to evaluate 2 superovulation injection protocols [a multiple-injection FSH (F) and a one-injection FSH-eCG (F+E)] for their ability to increase recovery of oocytes using laparoscopic ovum pickup (LOPU) technique in 10 prepubertal Kiko does. Does were randomly assigned to 2 sequence groups (n=5). Period 1 initiated on 28 July 2017 and Period 2 on 12 August 2017. A CIDR device was inserted on Day 0 and removed after LOPU. On Day 6 of each period, 3mL of prostaglandin F2α was given IM to each doe. F-treated does received (IM) twice daily injections of 15, 12.5, 10, and 7.5mg of FSH, whereas F+E-treated does received (IM) 80mg of FSH and 300 international units (IU) of eCG. Thirty-six hours after injection, follicles were aspirated using LOPU. Treatment response measurements included total follicles observed, oocyte recovery rate, and blastocyst rate. Total follicles observed were higher (P=0.004) in F+E-treated does (305 follicles) than F-treated does (236 follicles). Neither oocyte recovery rates (F+E=41.5% and F=38.1%) nor blastocysts rates (F+E=18% and F=32%) were affected (P>0.05) by injection protocol. In conclusion, we summarise that multiple injections of FSH can results in a higher number of follicles produced; however, this does not have a subsequent effect on IVF and development rates.