Silver nanoparticles can be synthesized biologically by means of microbes and plants since they offer eco-friendly, non-toxic and uniform nanoparticles, though many researchers have done major work on biosynthesis there is very less research carried out on synthesis of nanoparticles by Panchakavya. Panchakavya is an Indian eco friendly fertilizer. Silver nitrate is treated with panchakavya to synthesize silver nanoparticles where in panchakavya acts as a reducing agent. The synthesized silver nanoparticles are monitored by UV-spectrophotometer then subjected to structural and morphological studies by XRD, SEM and AFM and silver nanoparticles of size 20–35 nm were obtained. The silver nanoparticles are then used against napkins containing urine sample for antimicrobial studies. Then the napkins are examined for the growth of colonies by carrying out bacterial culture method. The comparison study was done between the napkin without silver nanoparticles and with silver nanoparticles by using colony counting process. The antibacterial activity of silver nanoparticles were traced for two weeks and found same activity which was shown initially. Hence antimicrobial activity of AgNPs lasted even after weeks in napkin used.