We have determined the comparative efficacy of the effect of cuprum and cobalt nano-particles (30-40 nm), humic acids in an ultra-fine state and mineral salts of these metals used as micro-fertilizers on plants growth and development at concentration from 0.01 till 1000 gr. per hectare norm of viability (gr./h.n.v.). We have conducted some bio-chemical investigations and studied activity of peroxydase and superoxide dismutase ferments in wheat roots and seedlings.The lab tests have determined concentrations when spring wheat plantlets growth and development slows down: for cuprum nano-particles -500 gr./h.n.v., cobalt -400 gr./h.n.v., copper sulphate and cobalt chloride -100 gr./h.n.v. The concentrations of 0.5-10 gr./h.n.v. cuprum and cobalt nano-powders have a well defined stimulating effect. The effect of using the metals nano-particles has been 8-12 % higher than that of the humic acids. Nano-particles have energy to activate metabolic cell processes of plants and concentrations up to 10 gr./h.n.v. can be recommended for the use in agrotechnology that is proved by field tests: wheat yield has increased under the humic acids per 7 %, cuprum and cobalt nano-particles per 17 % and 14 % correspondingly as compared with the control.Cuprum and copper oxide nano-powders (of different chemical substance) at concentrations 0.1 gr./h.n.v.-100 gr./h.n.v. have influenced activity of phytohormones of cytokinin, gibberellin, abscisic acid and indole acetic acids in wheat seedlings. The increase of cuprum nano-powder concentration in 100 times has not caused the decrease of wheat physiologic processes. For copper oxide nano-powders at concentration of 100 gr./ha the hormones activity changes from 22 to 53 % and the use of copper oxide at concentrations higher than 100 gr./h.n.v. is unreasonable. The wheat seeds treatment with 200 nm particles before planting has not changed morpho-physiologic and biochemical indexes as related to the control.