Long-term tillage and fertilization system have a strong impact on earthworms biomass and abundance in agriculture soils, however, enumerating their influence on mungbean plantation remains little studied. The purpose of this research was to study the effect of continous tillage and fertilization and their interaction on earthworms biomass and abundance under mungbean plantations. Two factors of treatments consisted of tillage system (minimum tillage and intensive tillage) and fertilization (without fertilization and fertilization by NPK (15:15:15) + chicken manure) what has been done since the first (2017) to sixth planting season (2020). The handsorting method was conducted at vegetative maximum and harvest time of mungbeans. The results show that the abundance and biomass of earthworms are significantly higher in minimum tillage compared to intensive tillage as well as fertilization compare to without fertilization. The greatest earthworms biomass and abundance were found in the maximum vegetative of mungbean. The interaction effect occurred at the maximum vegetative observation, namely in intensive tillage, there was no effect of fertilization, on the contrary, at minimum tillage, fertilization increased the abundance and biomass of earthworms. As a consequence, our results indicate that minimum tillage and fertilization significantly improve soil biological quality.