The experiment entitled “Effect of varieties and spacing on vegetative growth and root yield of safed musli (Chlorophytum borivilianum)” was carried out to find out the suitable variety with optimum spacing 2018-19 and 2019-20 which was designed in factorial RBD with two varieties and three spacing levels. The trials were sown in two years i.e. on 02-07-2018 and 31-05-2019. The present investigation has been carried out at the main experiment station, Department of Horticulture at Medicinal and Aromatic Plant, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya, Uttar Pradesh, India. The data has been recorded in the following parameters, like leaf length, leaf width, number of tillers/hill, length and diameter of fingers, number of fingers per plant, fresh root yield, and dry root yield. It is effective against male sexual disorders such as erectile dysfunction, premature ejaculation, and infertility and is used as a health tonic.

Malnutrition is a very big issue in poor nations, particularly in Asia and Africa, where millions of pre-school-aged children with pregnant women suffer from it. Because they eat a carbohydrate-rich but micronutrient-scant plant-based diet, poor individuals are more vulnerable to malnutrition and hidden hunger. The spread of high-yielding varieties but low-micronutrient cultivars exacerbated the malnutrition. Supplementation & food fortification of staple foods with minerals are two ways that can be used to track the issue of proper nutrition safety. However, fortified and dietary supplements are also not possible or cost-effective for iodine, particularly iron. To treat micronutrient deficiencies, genetic biofortification of crops has recently developed as a self-targeted and non-recurrent strategy. Because there wasn't enough genetic variation in the crossable gene pools, most traditional breeding methods were limited. Furthermore, it lacks the micronutrient and iodine accumulation-related modulation of target gene expression. At this point, genetic engineering-based food biofortification appears to be a potential strategy to solve hidden hunger, particularly in areas where breeding is difficult due to a scarcity of genetic diversity. If there is insufficient genetic variability and fixable major gene effects, genetic engineering will be a viable option for enhancing micronutrients at targeted levels. Transgenic technologies are one method that can be used to improve genotypes by changing specific metabolic pathways with different genes.