Conventional photodynamic treatment strategies are based on the principle of activating molecular oxygen in situ by light, mediated by a photosensitizer, which leads to the generation of reactive oxygen species and thereby causes cell death. A diarylethene-derived peptidomimetic is presented that is suitable for photodynamic cancer therapy without any involvement of oxygen. This light-sensitive molecule is not a mediator but is itself the cytotoxic agent. As a derivative of the cyclic amphiphilic peptide gramicidin S, the peptidomimetic exists in two thermally stable photoforms that are interconvertible by light of different wavelengths. The isomer generated by visible light shows much stronger toxicity against tumor cells than the UV-generated isomer. First in vivo applications are demonstrated on a tumor animal model to illustrate how the peptidomimetic can be administered in the less toxic form and then activated locally in a solid tumor by visible light.

Conventional photodynamic treatment strategies are based on the principle of activating molecular oxygen in situ by light, mediated by a photosensitizer, which leads to the generation of reactive oxygen species and thereby causes cell death. A diarylethene‐derived peptidomimetic is presented that is suitable for photodynamic cancer therapy without any involvement of oxygen. This light‐sensitive molecule is not a mediator but is itself the cytotoxic agent. As a derivative of the cyclic amphiphilic peptide gramicidin S, the peptidomimetic exists in two thermally stable photoforms that are interconvertible by light of different wavelengths. The isomer generated by visible light shows much stronger toxicity against tumor cells than the UV‐generated isomer. First in vivo applications are demonstrated on a tumor animal model to illustrate how the peptidomimetic can be administered in the less toxic form and then activated locally in a solid tumor by visible light.

We describe the state of the immune system at the late stage of 1,2-dimethylhydrazine (DMH)-induced colon cancer and after administration of placenta-derived multipotent cells (PDMCs

The mechanisms of VEGF-mediated effects on endothelial cells during cancer development and progression is not clear. In present study the biological effects of VEGF, VEGF-rich culture medium of peritoneal macrophages from mice with Lewis lung carcinoma were studied on MAEC cell line under conditions of unfed culture. We have shown that VEGF increased cell proliferation by the 5th day of culturing vs control and anti-VEGF-treated cells. This effect was associated with increased consumption of glucose and NO production by the 2nd day while decreased – on the 5th day of cell culturing. VEGF-mediated NO production was dependent on Ca2+ ions. Block of Ca2+-channels (LaCl3) had more pronounced inhibitory effect vs chelator of Ca2+ ions (EDTA). It was shown that peritoneal macrophages are the main suppliers of VEGF at tumor angiogenesis, as evidenced by the data obtained on model system of endothelial cells synchronized in G0/G1 phase.

Aim:The aim of study was to show the combined influence of nimotuzumab (NTM) and EGF on the proliferation, apoptosis by breast cancer cells line MCF-7. Methodology: Cell line was cultivated in DMEM under standart conditions. Nimotuzumab and EGF was added to the cells which reached 70-80% monolayer. Flow cytometry assay was conducted to determine cell division in the phases of cell cycle and apoptosis. Cell viability was assessed by MTT test and routine calculation cells used Gor'yaev chamber test with trypan blue. Results: Obtained results show that the efficiency of the combined effects on tumor cells of breast cancer MCF-7 antibodies to epidermal growth factor receptor -nimotuzumab, in combination with epidermal growth factor enhances the cytotoxic/cytostatic effect of NTM. According to the data, NTM appeared a cytostatic, cytotoxic and proapoptotic effects. Thus, after NTM addition to cultured Original Research Article cells, relative quantity of cells in phases G0 / G1 were increased in 1.2 times (P<0.05), compared to control, the percentage of apoptotic cells was 4-fold increased (P<0.01), and dead cells -almost 2-fold (P<0.05). Previous incubation of cells with EGF (5 nM) during 2 hours before adding NTM was characterized by an increase in the relative content of cells in G0 / G1 phase to 78,3±2,2%, against 73,4±1,4% NTM impact, 58,8±2,1% in the control and 38,9±0,9% for EGF influence. There was also a redistribution of concentration of apoptotic and dead cells: reducing the percentage of apoptotic compared to the influence of the mono NTM and increase dead cells, respectively.