Lepomis gibbosus (L., 1758) (Pisces, Centrarchidae) is a native fish from North America whose introduction in Europe dates from around 1880 (Vooren, 1972). This exotic fish is nowadays widespread in European waters (Welcomme, 1991). According to García-Berthou & Moreno-Amich (2000a) the first confirmed record of the species in the Iberian Peninsula (Lake Banyoles) dates to between 1910 and 1913. Until the 1980's the species remained under control although since then it has spread for a high number of hydrological basins in the Iberian Peninsula (De-Sostoa et al.,1987;Doadrio, 2002).The aim of this note is to report the establishment of L. gibbosus in the Segura river basin. A total of 79 specimens were collected in seven out of a total of 15 sampling sites on the Segura River. The sample sites were located between Las Minas (upstream, Albacete 30SXH427557) and Azud de Ojós (downstream, Murcia 30SXH510469) (70 Km channel long). At each sampling locality (100-150 m long), fishing was performed with standard AC electrofishing equipment using a 1800 W generator (working voltage between 200 and 350 V, 2-3 A) between May and June 2004. The specimens were fixed in 199 Este trabajo constituye la primera cita de Lepomis gibbosus (L.,1758) en la Cuenca del río Segura (SE, España). La presente
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