ABSTRACT. Berberine (BBR) is a natural alkaloid with significant anti-tumor activity against many types of cancer cells. In this study, we investigated the molecular mechanisms employed by BBR to repress the proliferation and growth of skin squamous cell carcinoma A431 cells. Berberine was reported to inhibit the proliferation of A431 cells in a dose-and time-dependent manner and was observed to induce a series of biochemical events, including the loss of mitochondrial membrane potential, release of cytochrome-c to cytosol, induction of proteins of the Bcl-2 family and caspases, and the cleavage of poly(ADP)-ribose polymerase. This suggested its ability to induce apoptosis. The results of a wound healing test revealed that berberine inhibited the migration of A431 cells. Ezrin was transfected into A431 cells by RNA interference. The level of expression of Ezrin in the transfected A431 cells was observed to decrease with berberine treatment, which suggested that berberine might inhibit the invasion of A431 cells through Ezrin. The results of this study 10553-10568 (2015) demonstrated that berberine could potentially inhibit proliferation, induce apoptosis, and inhibit the invasion of A431 cells.

In the operation of the imported container area of the container yard, the fault of the yard crane often occurs, and the fault is random and unpredictable, which greatly affects the operational efficiency of the container yard. To improve the operation efficiency of the container yard, this paper studies the rescheduling optimization problem of the multi-container area and multi-yard crane when random faults occur in container lifting operations in the container import area. Considering the different impacts of different fault conditions on the container yard operation, the fault impact judgment mechanism is established. The waiting time of external container trucks and customer satisfaction is considered for yard crane rescheduling. Yard crane rescheduling model after the fault is constructed, aiming at the minimum deviation from the original scheduling scheme. And the AEA (annealing evolution algorithm) algorithm is used to solve it. The effectiveness of magic and the specificity of the algorithm are verified by the analysis of numerical examples in different scales. The research data of Dalian Port is used to carry out experiments, and the experimental analysis of examples in different scales verifies the effectiveness of the model and the scientific nature of the algorithm. Compared with the existing scheme, this scheme is more practical, which can not only give the treatment scheme immediately when the fault occurs but also effectively improve the working efficiency of the container yard and provide a reference for the port to enhance customer satisfaction.