Forkhead transcription factor family O (FoxO) maintains adult stem cell reserves by supporting their long-term proliferative potential. MicroRNAs (miRs) regulate neuronal stem/progenitor cell (NSPC) proliferation and differentiation during neural development by controlling the expression of a specific set of target genes. In the neurogenic subventricular zone, FoxO1 is specifically expressed in NSPCs and is no longer detected during the transition to neuroblast stage, forming an inverse correlation with miR-9 expression. The 3′-untranslated region of FoxO1 contains a conserved target sequence of miR-9 and FoxO1 expression is coordinated in concert with miR-9 during neuronal differentiation. Our study demonstrates that FoxO1 contributes to NSPC fate decision through its cooperation with the Notch signaling pathway.

Many researches have been reported on the condition monitoring of civil infrastructures by means of piezoelectric sensors. Most of them made use of the impedance change of the piezoelectric device in relation to the creation of internal damages to the structure. The impedance measurement is a well accepted method in the piezoelectric sensor area, and has been proved by many authors to be useful for civil structure diagnosis. However, the impedance measurement normally requires sophisticated equipment and analysis technology. For more general and wide application of the piezoelectric diagnosis tool, a new methodology is desired to overcome the limitations of the impedance measurement. This paper presents the feasibility of a piezoelectric oscillator sensor to detect the damages in civil infrastructures. The oscillator sensor is composed of an electronic feedback oscillator circuit and a piezoelectric thickness mode vibrator to be attached to the structure of interest. Damage to the structure causes a change in the impedance spectrum of the structure, which results in a corresponding change of the resonant frequency of the structure. The oscillator sensors can instantly detect the frequency change in a very simple manner. Feasibility of the piezoelectric oscillator sensor was verified in this work with a sample aluminum plate where artificial cracks of different depth were imposed in sequence. Validity of the measurement was confirmed through comparison of the experimental data with the results of finite element analyses of the plate with cracks. Performance of the oscillator sensor was also compared with that of its conventional counterpart, i.e. impedance measurement, to manifest the superiority of the oscillator sensor.