Porcine teschovirus (PTV), Porcine sapelovirus (PSV) and Enterovirus G (EV-G) have been associated with enteric, respiratory, reproductive and neurological disorders. Although Brazil is the world's fourth largest producer and exporter of pork, no information on the occurrence of PTV, PSV and EV-G infections is available for Brazilian pig herds. This study aimed to investigate the occurrence of Porcine enteric picornavirus infections in pig farms located in three distinct geographical regions of Brazil. Forty randomly selected diarrhoeic and normal consistency faeces of suckling (n = 22) and nursery (n = 18) pigs from farms located in 21 distinct cities of the Southern, Southeast, and Midwest regions of Brazil were evaluated by nested-RT-PCR assays. Suckling piglets presented the expected amplicon size for PTV (158 bp) and EV-G (313 bp) in single and mixed infections in 40.9 % (9/22) of the faecal samples. PSV amplicon (212 bp) was not detected in this age group. For nursery pigs, Porcine enteric picornaviruses amplicons were present in 77.8 % (14/18) of the faecal samples. PTV and EV-G were detected in single and mixed infections, while PSV was detected only in two samples in co-infection with PTV and EV-G in this age group. The Brazilian regions evaluated presented at least two of the tested viruses. Sequencing analysis revealed high similarities to the related viruses (95.3 to 99.2 % for PTV, 94.2 to 98.5 % for PSV and 86 to 100 % for EV-G). For the first time PTV, PSV and EV-G have been molecularly detected and characterised in pig faecal samples in Brazil.

Um dos principais problemas sanitários da suinocultura é a alta prevalência de doenças respiratórias, que podem ser causadas por uma série de agentes bacterianos e virais. Visando identificar os agentes bacterianos causadores das lesões pulmonares que geram condenação de carcaça de suínos, os pulmões de 150 animais em idade de abate foram submetidos a exames anatomopatológicos e bacteriológicos. Foram escolhidas as lesões pulmonares que causaram o desvio das carcaças na linha do abate para o Departamento de Inspeção Final do Serviço de Inspeção Federal. Pelo exame macroscópico as lesões foram classificadas em broncopneumonia supurativa, broncopneumonia fibrinosa, pleurite ou pneumonia embólica. Na pesquisa bacteriológica isolou-se Pasteurella multocida Tipo D (27,3%), Pasteurella multocida Tipo A (24%), Actinobacillus pleuropneumoniae (13,3%), Streptococcus suis (6,7%), Arcanobaterium pyogenes (5,3%) e outras bactérias (10,0%). Dentre o total de amostras, 16,7% foram negativas.  As lesões de onde se isolou P. multocida e A. pleuropneumoniae foram classificadas, em sua maioria, como broncopneumonia fibrinosa ou sequelas desta lesão. Streptococcus sp e A. pyogenes relacionaram-se com pequenos abscessos, em lesões com características de pneumonia embólica ou broncopneumonia supurativa.

RESUMO.-[Estudo molecular de teschovírus suíno, sapelovírus suíno e enterovírus G em javalis (Sus scrofa scrofa) de cativeiro no Paraná.] Teschovírus suíno (PTV), sapelovírus suíno (PSV) e enterovírus G (EV-G) são agentes infecciosos específicos da espécie suína que estão endemicamente disseminados em todo o mundo. O objetivo deste estudo foi investigar a infecção natural por estes picornavírus entéricos suínos em javalis (Sus scrofa scrofa) do estado do Paraná, Brasil e avaliar pecaris (Pecari tajacu e Tayassu pecari) como hospedeiros alternativos para estes vírus. Amostras fecais (n=36) de javalis assintomáticos (n=22) com idades de 2 a 7 meses (jovens, n=14) e 2 a 4 anos (adultos, n=8) e de pecaris (6 a 8 meses de idade, n=14) foram coletadas em um cativeiro e zoológico, res- Porcine teschovirus (PTV), porcine sapelovirus (PSV), and enterovirus G (EV-G) are infectious agents specific to pig host species that are endemically spread worldwide. This study aimed to investigate the natural infection by these porcine enteric picornaviruses in wild boars (Sus scrofa scrofa) of Paraná state, Brazil, and to evaluate peccaries (Pecari tajacu and Tayassu pecari) as alternative host species for these viruses. Fecal samples (n=36) from asymptomatic wild boars (n=22) with ages ranging from 2 to 7 months old (young, n=14) and 2 to 4 years old (adult, n=8) and from peccaries (6 to 8 months old, n=14) were collected from a farm and a zoo, respectively, both located in Paraná state. Reverse transcription-polymerase chain reaction (RT-PCR) and nested-PCR (n-PCR) assays targeting the 5'non-translated region of the virus genome were used for screening the viruses. Porcine enteric picornaviruses were detected in 12 out of the 22 wild boar fecal samples. According to each of the viruses, EV-G was most frequently (11/22, 50%) detected, followed by PTV (10/22, 45.5%) and PSV (4/22, 18.2%). Regarding the age groups, young wild boars were more frequently (9/14, 64.3%) infected with PTV, PSV, and EV-G than adult animals (3/8, 37.4%). One n-PCR amplified product for each of the viruses was submitted to sequencing analysis and the nucleotide sequences were compared with the related viruses, which showed similarities varying from 97.7% to 100% for PTV, 92.4% to 96.2% for PSV, and 87

The endemic character of urinary infections (UI) in sows makes collective antimicrobial therapies via feed a routine. This, however, generates sub-doses unable to heal and contribute to the selection of antibiotic-resistant bacteria. The use of individual therapy is the most appropriate procedure to be performed on animals with UI. With this study, we aimed to evaluate the occurrence of UI in sows housed in the western region of Paraná and the efficacy and cost-benefit of individual treatment. A total of 353 females were selected from five different herds, submitted to urine collection in the final third of pregnancy by spontaneous urination method. The samples were analyzed physically and chemically with the use of reagent strips, and the presence of nitrite was a determinant for positivity for UI. The animals with UI had urine submitted to a bacteriological evaluation, were treated with parenteral medication (marbofloxacin - single dose - 8 mg/kg), and submitted to a new urine collection 24h and 48h after the first. UI was observed in 4.53% of the females evaluated (16/353). Escherichia coli and Streptococcus sp. were the most frequently isolated agents. Seven days after the use of marbofloxacin 87.5% (14/16) of the animals were negative for UI, which demonstrates the efficacy of UI parenteral control. The diagnosis associated with individual therapy at the expense of collective medication was highly cost-effective, made it possible to drastically reduce the number of medicated animals, and was efficient in controlling UI. Thus, it is concluded that it is possible to make rational use of antibiotics by treating only sows that are proven to be positive for UI. This reduces the number of unnecessarily medicated animals and reduces the cost due to the use of antimicrobials only in sick animals.

Swines raised in intensive systems are highly susceptible to claw lesions. Moderate to severe lesions trigger an inflammatory response, causing pain and impairing reproductive and economic performance. Foot lesions in sows can cause considerable losses owing to reduced fertility and herd longevity. Proper diagnosis is an important step in correcting this problem; however, the seriousness of the impact of these lesions on swine herds remains unclear to most farm owners. Health monitoring has become an essential tool for veterinarians because it enables the detection of disease incidence and severity. In this study, we assessed claw lesions in sows reared under an intensive system to ascertain the prevalence and severity of lesions in Brazilian herds. The hind limb claws of 2,660 sows from 30 farms were examined for the following lesions: heel overgrowth and erosion (HOE), heel-sole cracks, white line lesions (WL), horizontal and vertical cracks in the toe wall, overgrown toes, and overgrown or missing dew claws. Claws were classified as normal (score = 0), mild (score = 1), moderate (score = 2), or severe (score = 3). At least one type of lesion was observed in 99.1% of the sows, whereas 29.7% displayed severe lesions. HOE was the most common lesion (89.9% of sows) and severe WL was observed in 16.8% of the sows. Lesions increased in prevalence and severity with parity, except for WL In young females, lesions on the volar surface were more common than those on cracked walls or overgrown toes, which should be considered when selecting gilts for breeding. A high prevalence of claw lesions was observed in Brazilian sows. Monitoring of these lesions is fast and simple. Regular monitoring provides information on claw health in a herd over time, allowing us to take measures to control and treat claw lesions, avoiding worsening of the problem, early culling of animals, and the associated productive and economic losses.