The main purpose of this study is to examine how risk perception is influenced by the type of disaster (flood or landslide) and victim characteristics. The data reported here are based on the National Risk Perception Survey (NRPS) that was administered for the victims and the general public in Taiwan in 2004. In that year, many towns in Taiwan were seriously affected by floods and landslides, resulting in huge economic losses and fatalities. The primary findings are: (1) the victims and the general public are concerned about the different potential hazards that might affect their residential area, (2) the negative associations between the sense of controllability and the perceived impact is high for landslide victims, but not for flood victims, and (3) disaster type, gender, and previously experienced disasters are good predictors of victims' attitudes toward natural disasters.

The aim of this study was to develop a technique to decellularize a porcine cartilage bone construct with view to using this as a biological scaffold for cartilage substitution. The decellularization protocol applied freeze/thaw cycles; this was followed by cyclic incubation in hypotonic tris buffer and 0.1% (w/v) sodium dodecyl sulfate in hypotonic buffer plus protease inhibitors. Nucleases (RNase and DNase) were used to digest nucleic acids followed by disinfection using 0.1% (v/v) peracetic acid. Histological analysis confirmed the absence of visible cells within the decellularized tissue. DNA analysis revealed the near-complete removal of genomic DNA from the decellularized tissues. The decellularization process had minimal effect on the collagen content of the cartilage. However, there was a significant reduction in the glycosaminoglycan content in the decellularized tissues. There was no evidence of the expression of the major xenogeneic epitope, galactose-α-1,3-galactose. Biomechanical indentation testing of decellularized tissues showed a significant change in comparison to the fresh cartilage. This was presumed to be caused by the reduction in the glycosaminoglycan content. Biocompatibility of the acellular scaffold was determined using contact cytotoxicity assays and a galactosyltransferase knockout mouse model. Decellularized porcine cartilage tissue was found to exhibit favorable compatibility in both in vitro and in vivo tests. In conclusion, this study has generated data on the production of an acellular cartilage bone matrix scaffold for use in osteochondral defect repair. To our knowledge, this is the first study that has successfully removed whole cells and α-gal from xenogeneic cartilage and bone tissue.

No abstract