Cytochrome
P450s (P450s) are key enzymes in the synthesis of bioactive
natural products in plants. Efforts to harness these enzymes for in vitro and whole-cell production of natural products have
been hampered by difficulties in expressing them heterologously in
their active form, and their requirement for NADPH as a source of
reducing power. We recently demonstrated targeting and insertion of
plant P450s into the photosynthetic membrane and photosynthesis-driven,
NADPH-independent P450 catalytic activity mediated by the electron
carrier protein ferredoxin. Here, we report the fusion of ferredoxin
with P450 CYP79A1 from the model plant Sorghum bicolor, which catalyzes the initial step in the pathway leading to biosynthesis
of the cyanogenic glucoside dhurrin. Fusion with ferredoxin allows
CYP79A1 to obtain electrons for catalysis by interacting directly
with photosystem I. Furthermore, electrons captured by the fused ferredoxin
moiety are directed more effectively toward P450 catalytic activity,
making the fusion better able to compete with endogenous electron
sinks coupled to metabolic pathways. The P450-ferredoxin fusion enzyme
obtains reducing power solely from its fused ferredoxin and outperforms
unfused CYP79A1 in vivo. This demonstrates greatly
enhanced electron transfer from photosystem I to CYP79A1 as a consequence
of the fusion. The fusion strategy reported here therefore forms the
basis for enhanced partitioning of photosynthetic reducing power toward
P450-dependent biosynthesis of important natural products.
The last decade has seen a range of studies using non-invasive neutron and X-ray techniques to probe the ultrastructure of a variety of photosynthetic membrane systems. A common denominator in this work is the lack of an explicitly formulated underlying structural model, ultimately leading to ambiguity in the data interpretation. Here we formulate and implement a full mathematical model of the scattering from a stacked double bilayer membrane system taking instrumental resolution and polydispersity into account. We validate our model by direct simulation of scattering patterns from 3D structural models. Most importantly, we demonstrate that the full scattering curves from three structurally typical cyanobacterial thylakoid membrane systems measured in vivo can all be described within this framework. The model provides realistic estimates of key structural parameters in the thylakoid membrane, in particular the overall stacking distance and how this is divided between membranes, lumen and cytoplasmic liquid. Finally, from fitted scattering length densities it becomes clear that the protein content in the inner lumen has to be lower than in the outer cytoplasmic liquid and we extract the first quantitative measure of the luminal protein content in a living cyanobacteria.
Ultrastructural membrane arrangements in living cells and their dynamic remodeling in response to environmental changes remain an area of active research but are also subject to large uncertainty. The use of noninvasive methods such as X-ray and neutron scattering provides an attractive complimentary source of information to direct imaging because in vivo systems can be probed in near-natural conditions. However, without solid underlying structural modeling to properly interpret the indirect information extracted, scattering provides at best qualitative information and at worst direct misinterpretations. Here we review the current state of small-angle scattering applied to photosynthetic membrane systems with particular focus on data interpretation and modeling.
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