Background
Oryza glumaepatula
represents an important resource of genetic diversity that can be used to improve rice production. However, hybrid sterility severely restricts gene flow between
Oryza
species, and hinders the utilization of distant heterosis in hybrid rice breeding.
Results
In order to fully exploit the beneficial genes of
O. glumaepatula
and facilitate the conservation of these gene resources, a set of chromosome single-segment substitution lines (SSSLs) was developed using an
indica
variety HJX74 as the recurrent parent and an accession of
O. glumaepatula
as the donor parent. During the process of SSSLs development,
S23
, a locus conferring hybrid male sterility between
O. sativa
and
O. glumaepatula
, was identified and fine mapped to 11.54 kb and 7.08 kb genomic region in
O. sativa
and
O. glumaepatula
, respectively, encoding three and two candidate ORFs, respectively. qRT-PCR and sequence analysis excluded one common
ORF
as the candidate gene. In addition, hybrid male sterility caused by
S23
was environment-sensitive, and could be observed only in natural short-day (NSD).
Conclusion
Identification and candidate genes analysis of
S23
in this study provides a valuable example to study the crosstalk between interspecific F
1
hybrid male sterility and environment-conditioned male sterility in rice, facilitates reserving and utilizing favorable genes or alleles of wild
Oryza
species, and allows for a more efficient exploitation of distant heterosis in hybrid rice breeding.
Electronic supplementary material
The online version of this article (10.1186/s12284-019-0271-4) contains supplementary material, which is available to authorized users.
Chemical and enzymatic modifications intended to improve the reactivity of dissolving pulp rapidly decrease its yield. In this study, a beating posttreatment intended to increase the reactivity of bamboo dissolving pulp was investigated. Beating post-treatment can create microfibrils on the surface of fibers. The reactivity of unrefined bamboo dissolving pulp prepared via pre-hydrolysis and a subsequent kraft cooking and Op-H-P (oxygen delignification enhanced with H 2 O 2 and sodium hypochlorite) bleaching process was very low. The reactivity increased drastically as the Canadian standard freeness (CSF) of the bamboo dissolving pulp was decreased (i.e., the degree of beating increased). The CSF decreased to 236 mL from its original, higher freeness. The average fiber width was larger and the curling and kink indexes were lower in the pulp of CSF 236 mL compared to those of pulps with greater freeness. However, there was little impact of beating on the crystallinity index of bamboo dissolving pulp.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.