For the present study, we have successfully fabricated a novel cylinder-shaped device that is able to produce mono-dispersed plasma bubbles (balls) under atmospheric pressure and water environment. The generated plasma has characteristics of low temperature, which was generated by dielectric barrier discharge. Multiple-electrodes were mounted to transport the plasma bubbles (balls) to the further downstream to provide larger radical effect to the biological target. It was confirmed that the plasma bubble has been successfully transported downstream with the distance of 18.7[mm]. This study will be combined to the endoscopic treatment and contribute to the therapeutic aims, biomedical processes and sterilization treatment.

We prepared the mixed monolayer-modified Au electrode composed of negatively charged Co 111 complex (1) and hydroxyl-terminated alkanethiols (C,; n = 2, 6), 1/C,-Au, using the "step-by-step" immobilization method; the low-d~nsity monolayer of 1 (1-Au) was firstly constructed with a spacing and then C, molecules were filled in the gap of 1. In the cyclic voltammetric measurement of horse heart cytochrome c (cyt c) using 1/C2-Au, only a pair of oxidation-reduction wave was observed with almost the same peak separation as the case of 1-Au. Since the electron transfer rate by using the single monolayer of~ was much faster than that of 1-Au, it clearly indicates that the modification ofC 2 does not cause the phase-separation and C 2 molecules on Au do not influence to the electron transfer reaction with cyt c. In contrast, 1/C.,-Au showed faster electron transfer rate than the cases of 1-Au and 1/C 2 -Au, but exhibited slower rate than that of single monolayer of C 6 • These findings indicate that cyt c frrstly interacts with negatively charged 1, and then electron transfer reaction proceeds via C 6 molecules on Au electrode.

For the present study, we have successfully fabricated a novel cylinder-shaped device that is able to produce mono-dispersed plasma bubbles under atmospheric pressure and water environment. The generated plasma has characteristics of low temperature, which was generated by dielectric discharge barrier. Also, the plasma bubble has been successfully transported downstream with distance of .[ ].

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