Since 2017, a zoonoses prevention and control programme has been implemented in four pilot districts in Indonesia adopting a One Health (OH) approach, involving officers from the public health, animal health, and wildlife sectors. After a series of trainings, coordination among sectors has been enhanced and disease information shared among all sectors and used to guide rabies risk mitigation efforts. The objective of the current study was to evaluate the OH capacity building program in the pilot districts of Minahasa, Boyolali, Ketapang and Bengkalis. The initiative was assessed using the questionnaire data collected from each district and utilizing a framework developed by the Network for Evaluation of One Health (NEOH). The OH-index is used as an estimation of the degree to which OH is integrated into the operations and supporting infrastructure of the initiative. The results of this assessment show that the zoonoses prevention and control programme in Indonesia incorporates effectively the OH approach, both in its operations and the associated infrastructure. According to the data collected in four pilot areas, the initiative has a OH-ness index score of 0.74. This number indicates that this programme is a good example of how OH can be implemented in Indonesia.