1485 Recombinant human granulocyte colony-stimulating factors (rhG-CSF) including filgrastim, lenograstim and pegfilgrastim are widely used to treat chemotherapy-induced neutropenia. However, it remains a challenge to manage severe neutropenia in cancer patients after high dose chemotherapy. The activation of G-CSFR by the G-CSF requires dimerization of two receptor chains bound to two G-CSF ligands. We hypothesized that a G-CSF dimer might generate a faster in vivo response, thus to benefit patients with severe neutropenia. F-627 is a recombinant human G-CSF dimer expressed in mammalian cells. In vitro, F-627 was able to activate STAT3 and to stimulate the proliferation of 32D-GCSFR and M-NSF60 cell lines. To evaluate the efficacy of F-627 in chemotherapy-induced neutropenia, 36 adult cynomolgus monkeys (3/sex) were injected with cyclophoshamide (CY, i.v., 60 mg/kg on day 0 and 65 mg/kg on day 1). Animals were randomized to receive (s.c.) either carrier, or F-627 on day 5 and day 10, at 25, 60 and 150 μg/kg, or rhG-CSF at 10 μg/kg /day (daily injection), or pegfilgrastim at 60 μg/kg on day 5 and day 10. Pharmacokinetics and pharmacodynamics (PK/PD) were evaluated. Significant absolute neutrophil count (ANC) increase was observed in animals treated with F-627 at 25 μg/kg compared to carrier-treated monkeys. At nadir, F-627 at 60 μg/kg generated optimal ANC response with 6.9- and 3.0-fold higher ANC compared to pegfilgrastim- (0.17 × 109/L) and rhG-CSF- (0.39 × 109/L) treated monkeys, respectively. The PK parameters of F627 at 60 ug/kg including MRT, Cmax, Tmax, AUC and CL were comparable to that of pegfilgrastim-treated monkeys, despite the relative G-CSF molar dose in F-627-treated animals was 2.5x lower than the pegfilgrastim-treated animals. The results demonstrate that the G-CSF dimer generated a faster response in CY-treated monkeys compared to the G-CSF monomer-treated animals, suggesting that F-627 could benefit cancer patients with severe neutropenia after high-dose chemotherapy. Disclosures: No relevant conflicts of interest to declare.

This thesis is the introduction of a certain Petro-chemical factory about the locked block hopper control system of reforming equipment. The equipment is controlled by the American TRICON control system completes together by American Honneywell DCS. installment's hard core - The catalyst regeneration unit and the unit interconnection part's control is completes in the TRICON control system, the data passes to the DCS control system through the serial port, the operators can monitor the situation. This design concept is applicable in the complex programmed control and operation in refinery.

In order to detect the surface defects of metal work piece, a sensor array is designed based on the principle of eddy current detection. According to the difference of frequency in LC oscillatory circuit, the information of the metal work piece can be obtained. In order to acquire a stable result, an algorithm is proposed to eliminate the transient disturbance and to reduce the influence of temperature drift. With the embedded system of arm7 kernel, the result of data can be displayed in graphical format. The experiment shows that the sensor array has soundness and operability.

In order to reduce the loss of an automatic coin machine for using a 1-Yuan false coin, an improved device is designed by applying the eddy current nondestructive testing technology, which uses two distinct frequency signals to detect the information of coin. The device includes four modules: two LC oscillating circuits, signal processing module, microprocessor module and external control circuits. The detection algorithm selects two counting values under two distinct frequency signals. The low frequency circuit detects the information of the coin material, and the high frequency circuit detects the feature of the coin surface. The detection region of true coin is decided by the method of least square. The experiment shows that the device has the characters of stability, reliability and high accuracy.

In order to provide road information for outdoor mobile robot in a complicated environment, a new roadside detection method is proposed based on obstacle detection by applying a four-layer laser radar LD_ML. Because roadside obstacles distribute alone a road, theirs fitting straight lines are parallel to the road. The roadsides detection algorithm includes four steps: first, judge if there are obstacles along roadside or not; second, extract obstacles which belong to roadsides; third, build fitting straight lines through the roadside obstacles; at last, in order to obtain steady and precise roadsides, a EKF method is performed to track the roadsides. The results of experiment have testified the road roadsides detection algorithm has high stability and reliability.