*Graphical Abstract (for review) Highlights:Alkyl substituted silane precursors were used for entrapment of β-D-galactosidaseThe highest activity in optimized immobilization conditions was 84 μmol minThe entrapped β-D-galactosidase demonstrated increased pH and temperature stabilityMore than 60% residual activity was preserved after 5 reutilizations transmission electron microscopy, scanning electron microscopy, fluorescence confocal 2 microscopy, and porosity measurement. The biocatalyst was successfully reused in five reaction 3 cycles, maintaining more than 60% of the initial activity. 4