No abstract

Research to identify plant bioactive compounds led to the evolution of extraction methods. This study optimized ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) to extract the flavonoids of Croton grewioides Baill. species contributing to characterize the polar extract profile and understand the antioxidant potential of these plant constituents. The use of experimental design and statistical treatments enabled the determination of the ideal conditions for each technique. The extracts of five accessions of C. grewioides were obtained under the optimized extraction conditions and were analyzed using ultra high-resolution mass spectrometry (FT-Orbitrap MS) operating at negative ionization mode for flavonoids detection. The accurate experimental mass obtained to the main compounds was used to attribute the molecular formula. Chemical structures of the main compounds detected were proposed using structure data bases. Chemometric analysis were performed with two FT-Orbitrap MS spectra samples using the identified metabolites and, the antioxidant activity data, showing that for this species the MAE was most effective in extracting the antioxidant compounds. It was possible to propose the structures for forty compounds in the C. grewioides extracts, demonstrating the excellent performance of the FT-Orbitrap MS in providing information on the chemical profile of polar compounds in plant extracts.

No abstract

No abstract

Depression is a complex and multifactorial neuropsychiatric disorder that affect 4% of the world's population. The main hypothesis that underlies its pathophysiology is about neuronal dysfunctions, with a focus on monoamines dysregulation. However, recent studies have shown disturbances in oligodendrocytes and myelination in the pathophysiology of disease. Treatment for depression is mainly based on the use of antidepressants that modulate the levels of monoamines and their receptors, but their effects on oligodendrocytes are still scarce. Thus, the aim of this study is to investigate the proteome of a human oligodendrocyte cell line (MO3.13), treated with three different classes of antidepressants, in order to unreveal pathways and biological processes that could be implicated or associated with the pathophysiology and treatment of this disorder.