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This paper presents data on reactions of murine macrophages with a variety of lectins, with special focus on Griffonia simplicifolia I-B4 isolectin, the only lectin we tried that distinguishes stimulated macrophages from resident populations. Specificity of Gniffonia simplicifolia I reaction with carbohydrate determinants at the cell surface is shown by (i) ability of a-galactosidase treatment of intact cells to abolish all lectin binding whereas .-galactosidase has no effect on lectin binding, (ii) ability ofmethyl a-D-galactopyranoside to completely inhibit lectin binding with methyl a-D-glucopyranoside having no effect on lectin binding, (iii) ability of brief treatment of intact cells with trypsin to liberate a glycopeptide that reacts with G. simplicifolia I to form a precipitate that is dissolved by addition ofmethyl a-D-galactopyranoside or a-galactosidase but not by addition of methyl a-D-glucopyranoside or /3-galactosidase, (iv) ability of methyl a-D-galactopyranoside (but no other monosaccharide) to completely inhibit avid binding of macrophages to G. simplicifolia I lectin immobilized on an insoluble support, and (v) ability of immobilized lectin to separate macrophages into highly pure subpopulations of lectin-reactive and lectin-unreactive cells, as shown by examination offluorescein-labeled lectin-treated cells with phase-contrast/ fluorescence microscopy. During investigations on the surface glycoproteins of the Ehrlich ascites tumor cell and their interactions with lectins, it was observed that intraperitoneal injection of Griffonia simplicifolia § lectin I (GSI) at the time of intraperitoneal inoculation with Ehrlich tumor gave virtually complete protection of the animal against growth of the tumor. This paper reports on one phase of subsequent work directed at elucidation of the mech-

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