We review the principle and methodology of leakage radiation microscopy (LRM) applied to surface plasmon polaritons (SPPs). Therefore we first analyse in detail the electromagnetic theory of leaky SPP waves. We show that LRM is a versatile optical far-field method allowing direct quantitative imaging and analysis of SPP propagation on thin metal films. We illustrate the LRM potentiality by analyzing the propagation of SPP waves interacting with several two dimensional plasmonic devices realized and studied in the recent years.PACS numbers:

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Research on antimicrobial peptides is in part driven by urgent medical needs such as the steady increase in pathogens being resistant to antibiotics. Despite the wealth of information compelling structure-function relationships are still scarce and thus the interfacial activity model has been proposed to bridge this gap. This model also applies to other interfacially active (membrane active) peptides such as cytolytic, cell penetrating or antitumor peptides. One parameter that is strongly linked to interfacial activity is the spontaneous lipid curvature, which is experimentally directly accessible. We discuss different parameters such as H-bonding, electrostatic repulsion, changes in monolayer surface area and lateral pressure that affect induction of membrane curvature, but also vice versa how membrane curvature triggers peptide response. In addition, the impact of membrane lipid composition on the formation of curved membrane structures and its relevance for diverse mode of action of interfacially active peptides and in turn biological activity are described. This article is part of a Special Issue entitled: Interfacially Active Peptides and Proteins. Guest Editors: William C. Wimley and Kalina Hristova.

We report the realization of two-dimensional optical wavelength demultiplexers and multiports for surface plasmons polaritons (SPPs) based on plasmonic crystals, i.e., photonic crystals for SPPs. These SPP elements are built up of lithographically fabricated gold nanostructures on gold thin films. We show by direct imaging of laterally confined SPP beams in the visible spectral range by leakage radiation microscopy that SPPs of different wavelengths are efficiently rerouted into different directions. In addition we demonstrate the generation of three output SPP beams from one input beam.

We present a temperature-regulated, alphavirus replicon-based DNA expression system. The system is regulated by a viral temperature-sensitive RNA-dependent RNA replicase, creating a temperature-dependent RNA amplification loop. Because of this positive feedback, the system exhibits both low background and high inducibility. We observed 700-fold induction in transiently transfected cells, and over 104-fold induction in stably transfected cells. The high stringency of inducibility allowed the generation of stable cell lines expressing a highly toxic protein upon temperature shift. These data suggest that the present expression system could simplify bioprocess engineering strategies, especially in situations where the cloned protein has detrimental effects on host cell metabolism.