A novel conotoxin, named as PiVIIA, was isolated from the venom of Conus princeps, a marine predatory cone snail collected in the Pacific Southern Coast of Mexico. Chymotryptic digest of the S-alkylated peptide in combination with liquid chromatography coupled to tandem mass spectrometry, were used to define the sequencing of this peptide. Eleven N-terminal amino acids were verified by automated Edman degradation. PiVIIA is a 25-mer peptide (CDAOTHYCTNYWγCCSGYCγHSHCW) with six cysteine residues forming three disulphide bonds, a hydroxyproline (O) and two gamma carboxyglutamic acid (γ) residues. Based on the arrangement of six Cys residues (C-C-CC-C-C), this conotoxin might belong to the O2-superfamily. Moreover, PiVIIA has a conserved motif (-γCCS-) that characterizes γ-conotoxins from molluscivorous Conus. Peptide PiVIIA has 45% sequence identity with γ-PnVIIA—the prototype of this family. Biological activity of PiVIIA was assessed by voltage-clamp recording in rat dorsal root ganglion neurons. Perfusion of PiVIIA in the µM range produces a significant increase in the Ca2+ currents, without significantly modifying the Na+, K+ or proton-gated acid sensing ionic currents. These results indicate that PiVIIA is a new conotoxin whose activity deserves further studies to define its potential use as a positive modulator of neuronal activity.

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La Anquiloglosia parcial se define como una limitación de las posibilidades de protrusión y elevación de la punta de la lengua por causa de un frenillo lingual corto. El objetivo de este artículo es reportar el caso de anquiloglosia incompleta de un paciente pediátrico de 8 años de edad, el cual presenta problemas en la articulación de las palabras. La madre del paciente menciona que el niño recibió terapia de lenguaje en su escuela sin mostrar mejoría, por lo cual lo refirieron para atención odontológica. Se realizó el diagnóstico con éxito, donde se confirma la anquiloglosia y se decide realizar frenectomía, se recomienda continuar con la terapia de lenguaje.   Palabras clave: Anquiloglosia, frenillo lingual, frenectomía.