The recovery of ancient germplasm in\ud tomato (Solanum lycopersicon L.) has become necessary\ud to limit the wide genetic erosion caused by the\ud employment of modern cultivars. Among germplasm\ud collections, long shelf-life landraces could represent an\ud important source of biodiversity. The present study\ud provides a first set of molecular and phenotypic data on\ud long shelf-life (so called ‘‘da serbo’’ in southern Italy)\ud tomato collection, mainly originated from Sicily\ud together with some landraces from Campania and\ud Apulia. The analysis of fruit traits showed a low intravarietal\ud variation, while exhibiting a quite higher intervarietal\ud variability. Overall, the cultivars have been\ud classified in six fruit shape classes of which flattened\ud and slightly flattened included the 54.76 % of the\ud collection. The principal component analysis (PCA)\ud showed a large cluster in which almost all landraces\ud from Sicily were included. The microsatellite (SSR)\ud analysis confirmed a low intra-varietal variation, and\ud the very low heterozygosity (Ho) revealed a high degree\ud of homozygosity in these landraces. In accordance with\ud limited morphological variability, the values of microsatellite\ud polymorphism (PIC) showed a low genetic\ud variability among these long shelf-life tomato cultivars.\ud Cluster analysis based on 10 polymorphic SSR was not\ud able to distinguish landraces for their different origin,\ud while allowed to classify similar genotypes in four\ud groups. Three groups showed a limited genetic distance\ud while in a fourth largest and genetic variable cluster was\ud included genotypes more selectable for traits of agronomic\ud interest. Overall, the phenotypic and genetic\ud variation allowed us to classify a collection of Sicilian\ud long shelf-life tomato landraces