Thermal lesions were produced in 12 male Wistar rats, positioning a massive aluminum bar 10 mm in diameter (51 g), preheated to 99°C ± 2°C/10 min. on the back of each animal for 15 sec. After 7, 14, 21, and 28 days, animals were euthanized. The edema intensity was mild, with no bubble and formation of a thick and dry crust from the 3rd day. The percentage of tissue shrinkage at 28 days was 66.67 ± 1.66%. There was no sign of infection, bleeding, or secretion. Within 28 days reepithelialization was incomplete, with fibroblastic proliferation and moderate fibrosis and presence of modeled dense collagen fibers. It is concluded that the model established is applicable in obtaining deep second-degree thermal burns in order to evaluate the healing action of therapeutic agents of topical use.

Thisstudy aimed at evaluating the use of lectin gel in the treatment ofsecond-degree burns in rats immunocompromised. Thirty-two male rats were randomly divided into two groups (G1 = treatment with hydrogel containing 100 μg / ml Cramoll 1,4 and G2 = Control, hydrogel without lectin). Thermal lesions were produced in the animals of both groups, positioning a massive aluminum bar 10 mm in diameter (51 g), preheated to 99° C ± 2° C/10 min in the dorsal proximal region for 15 sec. After 7, 14, 21 and 28 days, animals were euthanized. The percentage of tissue shrinkage in the group treated with lectin at 28 days was 81.0 ± 2.2 %. There was no sign of infection, bleeding or secretion. There were nosignificant differences inbiochemical and hematological parametersanalyzed.Histological evaluation of G1revealed: on the 7th day moderate inflammatory infiltrate and mild fibrosis, on the 14th d ay intense autolysis, neovascularization, mild fibroblast proliferation and intensefibrosis, on the 21st day reepithelialization, non-modeled and densecollagen, moderate fibrosis and on the 28 th day complete tissue epithelialization. These results extend the potential of therapeutic applications for Cramoll 1,4 in the treatment of thermal burns in immunocompromised animals.

No abstract

Milk is considered a nutritionally noble food and is therefore suitable for feeding children and adults. However, contamination of milk by mycotoxins may pose a health risk to the consumer. Aflatoxins, mycotoxins produced by fungi of the genus Aspergillus, can be found in several food products, including milk and its derivatives, which reinforces the importance of this type of study on the occurrence of the aflatoxin M 1 (AFM 1 ) in raw milk. We analyzed 45 samples of raw bovine milk from expansion tanks from 23 farms in different municipalities of the dairy belt of the state of Alagoas/Brazil. Samples were collected directly from the cooling tanks and transported under refrigeration for analysis. The method used for the extraction of AFM 1 was that proposed by the Adolfo Lutz Institute. On the other hand, the detection of AFM 1 occurred by high performance liquid chromatography (HPLC) by identifying retention times. The results of the analyses indicated that none of the collected samples presented contamination by aflatoxin M 1 , thus indicating that the milk commercialized in Alagoas shows a good quality against this toxic agent.

A produção brasileira de pinha (Annona squamosa L.) predomina no Nordeste, sendo afetada pela antracnose causada por Colletotrichum gloeosporioides. Este estudo avaliou: 1) as taxas de crescimento micelial e conidiação, dimensões de conídios e produção de amilase, xilanase, pectinases e protease por isolado desse fungo de lesões de abacate (Persea americana Mill), em diferentes meios; 2) as porcentagens de germinação e formação de apressórios do mesmo sobre folhas jovens de pinha; 3) as alterações in vivo nos teores de proteínas, fenóis e carboidratos solúveis totais, antes e após a inoculação. Folhas jovens de plântulas de dois ecótipos de pinha (PI e CT) foram destacadas, submetidas à inoculação e incubadas ou para sua extração (0 e 36 horas após), ou para seu clareamento (0, 12, 18, 24, 30, 36, 42 e 46 horas após), coloração e análise ao microscópio. Particionou-se cada extrato contra hexano, e a fração polar foi concentrada e resolubilizada para determinação dos parâmetros bioquímicos mencionados. Verificou-se maior esporulação do isolado fúngico em meio Mathur, e este produziu todas as enzimas ensaiadas in vitro. In vivo, este foi mais agressivo ao ecótipo PI, mas verificou-se ca. de 80% de germinação e 50% de formação de apressórios após 24 e 30 horas de incubação respectivamente sobre os ecótipos PI e CT. Os teores de proteínas, glicídeos redutores e fenóis totais dos extratos de CT foram mais elevados 36 horas após a inoculação, enquanto apenas uma ligeira elevação no conteúdo de fenóis foi constatada nos extratos de PI. Palavras-chave:Annona; Colletotrichum; pinha; antracnose; resistência bioquímica.