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Pre-incubation with 10 μM DCFDA for 30 min in PBS was sufficient to generate a sensitive and reproducible standard curve for detection of UVA-induced ROS in HaCaT and HPF cells, with no effects on cell viability or morphology.

Mônico, D.A. Synthesis of spheroidal models of melanomic cells at different degrees of progression associated with dermo-equivalent systems for treatment viaPhotodynamic Therapy. 2021. 94f. Thesis (Master's degree). Faculty of Philosophy, Sciences and Letters of Ribeirão Preto -