High molecular weight (1MW) and low molecular weight (LMW) forms of von Wiflebrand factor (vWF) were isolated from normal human plasma in the presence of protease inhibitors. HMW and LMW vWF preparations were subjected to reduction of interdimeric diside bridges under mild reducing conditions. Foilowing sodium dodecyl sulfate eectrophoresis in 3% agarose, the vWF bands were detected by immunoblotting with a polyclonal rabbit anti-vWF antiserum as well as with two monoclonal antibodies dircted against epitopes located in the NHrterminal (MAb 418)

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