To speed up the molecular analysis of cassava transgenic plants, we developed real-time polymerase chain reaction (PCR)-based methods that could be implemented as a tool in the primary scrutiny of putative transgenic plants. We tested for the presence of transgenes, estimated copy number, and quantified messenger RNA (mRNA) levels of genes introduced through Agrobacterium. Copy numbers for the genes ß-glucuronidase and hygromycin phosphortransferase were estimated in 15 transgenic lines.
23A major constraint for incorporating new traits into cassava using biotechnology is the 24 limited list of known/tested promoters that encourage the expression of transgenes in
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ResumenSe describen los elementos fundamentales que componen la radio cognitiva y se analiza la asignación de bandas espectrales. Dentro de los principales problemas en la inserción de nuevas aplicaciones y tecnologías inalámbricas, está la falta de espectro radioeléctrico para su asignación, debido a la manera ineficiente de distribución del espectro radioeléctrico disponible, que es actualmente asignado de forma estática. La radio cognitiva nace como un método que propone una solución a la problemática, gestionando dinámicamente el recurso. Una de las etapas que integran esta tecnología es la decisión de espectro, en la que se selecciona y asigna las bandas frecuenciales a partir de los requisitos de calidad de servicio de los usuarios cognitivos. Este artículo plantea una propuesta para el desarrollo de un proyecto de investigación que optimice el proceso de selección de canales reduciendo el tiempo de asignación para elevar su rendimiento.
Palabras clave: radio cognitiva, toma de decisiones espectrales, asignación estática, asignación dinámicaAbstract Fundamental elements of the cognitive radio and assignment of spectral bands are described and analyzed. Among the main problems in the insertion of new applications and wireless technologies is the lack of radio spectrum for allocation due to the inefficient distribution of available radio spectrum, which is statically allocated at present. The cognitive radio paradigm was born as a method for proposing a solution to the problem, dynamically managing the resource. One of the stages that make up this technology is the decision of the spectrum that selects and allocates frequency bands based on quality of service requirements of the cognitive users. This article presents a proposal for the development of a research project that optimizes the selection of channel allocation by reducing the time to improve their performance.
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