In vitro physiology and carbon metabolism can be affected by the sink-source relationship. The effect of different sucrose concentrations (10, 30, and 50 g L −1 ), light intensities (80 and 150 μmol m −2 s −1 ), and CO 2 levels (375 and 1,200 μmol mol −1 ) were tested during plantain micropropagation in temporary immersion bioreactors. Activities of pyruvate kinase, phosphoenol pyruvate carboxylase, and the photosynthesis rate were recorded. From the morphological and practical point of view, the best results were obtained when plants were cultured with 30 g L −1 sucrose, 80 μmol m −2 s −1 light intensity, and 1,200 μmol mol −1 CO 2 concentration. This treatment improved leaf and root development, reduced respiration during in vitro culture, and increased starch level at the end of the hardening phase. In addition to that, the number of competent plants was increased from 80.0% to 91.0% at the end of the in vitro phase and the survival percentage from 95.71% to 99.80% during ex vitro hardening.
Las bromelias son plantas ornamentales muy atractivas por la coloración de sus hojas y la belleza de sus inflorescencias. Dentro de ellas las Vrieseas es uno de los géneros de mayor interés comercial. Cuando se desean introducir nuevos híbridos en el mercado, las técnicas de propagación tradicional son insuficientes, por lo que resultan de gran interés las técnicas de propagación in vitro. Con el objetivo de establecer un protocolo para la aclimatización de brotes de Vriesea propagados in vitro en Sistemas de Inmersión Temporal se evaluó el efecto de la aplicación del brasinoesteroide MH5 en la supervivencia y calidad de los brotes. La integración de los resultados constituyen los procedimientos elementales para un protocolo de aclimatización de brotes de Vriesea propagados con el empleo de la técnica de inmersión temporal.
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