Despite growing interest in using phylogenetic and functional methods to understand community assembly, few studies have examined how these methods can be used to assess seasonal variation in assembly mechanisms among migrant species.Migration can rapidly alter the relative influence of stochastic processes, species interactions, or environmental factors in shaping communities across seasons. Here, we describe seasonal dynamics in the phylogenetic and functional diversity of waterbirds in Mai Po Wetland, a subtropical region with significant and predictable temporal variation in climate and migratory bird density. Phylogenetic α diversity varied seasonally, exhibiting a clustered structure (indicative of environmental filtering) in summer, and over-dispersed structure (indicative of biotic filtering) in winter.Phylogenetic diversity in spring and autumn exhibited a more intermediate, random structure, consistent with stochastic arrivals and departures of migrants. Functional diversity was clustered in spring but showed over-dispersion in the other three seasons. Phylogenetic β diversity in summer and winter assemblages was characterized by two distinct groups, while spring and autumn assemblages were mixed. Our results suggest that waterbird assemblages were primarily shaped by interspecific competition in winter, while random processes tended to shape assemblages in spring and fall. Environmental factors played a more important role in summer, during periods of high heat stress. In addition, species co-occurrence patterns were significantly more strongly related to phylogenetic similarity in winter than in summer. Our results suggest that the relative importance of assemblage mechanisms can vary seasonally in response to changing environmental conditions, suggesting that studies attempting to infer a single dominant assembly mechanism may ignore important assembly processes. Temporal shifts in assembly mechanisms may play an important role in maintaining diversity in subtropical and temperate wetlands and perhaps also in other dynamic systems.

Urban expansion often accompanies a loss of natural habitats and arable lands but an increase in urban population. In China, vegetable-dominant small farmlands are common in urban/peri-urban areas. Some farmlands are also associated with government policy that aims to enhance local farmers’ livelihoods as well as increase food availability for city citizens. While small urban farmlands create open greenery cover that may provide birds with resources such as food and shelter, little attention has been given to understanding bird diversity in urban farmlands. Using two hierarchical models (multi-species occupancy model and N-mixture model), we examined how species richness and abundance of birds were associated with environmental characteristics within and surrounding urban farmlands in Guangzhou, one of the largest cities in China. We conducted crop and bird surveys at urban farmlands during two winter seasons between December 2019 and January 2021. Species richness increased with non-woody (herbaceous) vegetation cover within a farmland. Abundance of three species was also positively associated with the local non-woody vegetation variable. Two species were more abundant at farmlands with higher crop diversity. Compositional features of matrix surrounding a farmland (a 500-m circular area) did not affect species richness. However, species richness and abundance of one species tended to decrease with increasing farmland fragmentation (patch density of farmlands) within a 1-km circular area. These findings suggest that (1) birds could be more influenced by environmental features within farmlands than matrix features surrounding farmlands, (2) local uncultivated herbaceous vegetation is an important environmental feature, and (3) diverse crops in farmlands may benefit some birds. They also indicate that the landscape pattern of farmlands, such as degree of fragmentation, could affect bird diversity in urban farmlands.