The purpose of this study was to determine the effect of sprint exercise and a detraining period on lipid peroxidation (malondialdehyde, MDA) and response of antioxidant system (ferric reducing ability of plasma (FRAP), uric acid, bilirubin and total protein). Forty-two male rats were divided randomly into two groups: sprint group (experimental) (n=24) and control group (n=18, without any training programme). The experimental group trained for 12 weeks, 3 times per week, with given intensities. Five trained rats experienced the detraining, so the effects of detraining were also studied (from the 8th to 12th weeks). Data were analysed using a twoway repeated ANOVA. A significant difference between the two groups was observed for MDA (p=0.022), FRAP (p=0.005) and bilirubin (p=0.002) but for total protein and uric acid no significant change occurred. In the experimental group, a significant difference was found through various assessments for MDA (p=0.001), FRAP (p=0.001), bilirubin (p=0.008) and uric acid (p=0.012).It can be concluded that this protocol of sprint exercise training causes adaptation in the antioxidant system and lipid peroxidation, but by detraining these results will be reversed.