The aim of this study was to assess how red-eared sliders (Trachemys scripta elegans) respond to anaesthesia itself and coelioscopy. For that purpose, the turtles were anaesthetised with ketamine–medetomidine or propofol, and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and the level of malondialdehyde (MDA) were determined by spectrophotometry. The possible genotoxic effects of the anaesthetic agents were estimated by comet assay. A total of 24 turtles were included in this study. The animals were divided into four groups according to the anaesthetic protocol and according to whether endoscopy would be performed. Significantly decreased activities of CAT were found only in the propofol group and in turtles undergoing coelioscopy. Both anaesthetic protocols induced significantly increased MDA levels, while no differences were observed after the intervention. A significant increase in GST activity was detected in turtles after both anaesthetic protocols, but after coelioscopy significant changes in GST activity were found only in the propofol group. However, no differences in SOD activity and no DNA damages were detected in either group. These findings suggest that ketamine–medetomidine may be more suitable anaesthetic agents in red-eared sliders than propofol.
Although Mycobacterium spp. often cause disease in domestic birds (chickens and companion birds), there are few data on avian tuberculosis in wild populations, especially in birds of prey. We describe here a case of a young adult female, free-living Eurasian griffon vulture ( Gyps fulvus) that was found dead. Granulomas were grossly evident in the lungs at autopsy, and tuberculosis was suspected. Ziehl–Neelsen staining revealed large numbers of intracellular acid-fast–positive bacteria within granulomas. Examination on Löwenstein–Jensen medium was negative, but mycobacteria growth indicator tube medium results were positive. For the molecular detection of Mycobacterium spp., the primer set IS901F and IS901R was used. Positive results were observed on gel electrophoresis, indicating the presence of Mycobacterium avium subsp. avium DNA. Although tuberculosis is not considered to be a common cause of death in wild birds, it undoubtedly deserves special attention because vultures are generally considered to be a species resistant to a large number of pathogens. Determination of the cause of death of griffon vultures is important for future conservation measures for this sensitive wild species.
This affiliation was entirely missing. 5. The Keywords were incorrectly presented. Correctly they read as Keywords: red-eared slider, turtle, anaesthesia, coelioscopy 6. In the 8th line of the Section "Anaesthetic protocols" the redundant text (SC) was inserted.Correctly, the section reads as Anaesthetic protocols Two different anaesthetic protocols were used. The first protocol was applied in Group KMC and Group KM and involved using ketamine (Ketamidor 10% Richter Pharma, Austria) 20 mg/kg and medetomidine (Domitor, Orion Pharma, Finland) 0.2 mg/kg, both mixed in a single syringe and administered intravenously (IV) in the subcarapacial venous plexus. The second protocol was applied in Group PC and Group P and involved the administration of propofol (Diprivan 10%, AstraZeneca UK Limited, United Kingdom) 10 mg/kg IV in the subcarapacial venous plexus. In Group KMC and Group KM,
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