In European and many African, Middle Eastern and Southern Asian populations lactase persistence (LP) is the most strongly selected monogenic trait to have evolved over the last 10,000 years 1 . While LP selection and prehistoric milk consumption must be linked, considerable uncertainty remains concerning their spatiotemporal configuration and specific interactions 2,3 . We provide detailed distributions of milk exploitation across Europe over the last 9k years using c. 7,000 pottery fat residues from >550 archaeological sites. European milk use was widespread from the Neolithic period onwards but varied spatially and temporally in intensity. Surprisingly, comparison of model likelihoods indicates that LP selection varying with levels of prehistoric milk exploitation provides no better explanation of LP allele frequency trajectories than uniform selection since the Neolithic. In the UK Biobank 4,5 cohort of ~500K contemporary Europeans, LP genotype was only weakly associated with milk consumption and did not show consistent associations with improved fitness or health indicators. This suggests other hypotheses on the beneficial effects of LP should be considered for its rapid frequency increase. We propose that lactase non-persistent individuals consumed milk when it became available, but that under particular conditions and microbiological milieux this was disadvantageous, driving LP selection in prehistoric Europe. Comparison of model likelihoods indicates that population fluctuations, settlement density and wild animal exploitationproxies for these driversprovide better explanations of LP selection than the extent of milk exploitation. These findings offer new perspectives on prehistoric milk exploitation and LP evolution.

The application of biomolecular techniques to archaeological materials from the Balkans is providing valuable new information on the prehistory of the region. This is especially relevant for the study of the neolithisation process in SE Europe, which gradually affected the rest of the continent. Here, to answer questions regarding diet and subsistence practices in early farming societies in the central Balkans, we combine organic residue analyses of archaeological pottery, taxonomic and isotopic study of domestic animal remains and biomolecular analyses of human dental calculus. The results from the analyses of the lipid residues from pottery suggest that milk was processed in ceramic vessels. Dairy products were shown to be part of the subsistence strategies of the earliest Neolithic communities in the region but were of varying importance in different areas of the Balkan. Conversely, milk proteins were not detected within the dental calculus. The molecular and isotopic identification of meat, dairy, plants and beeswax in the pottery lipids also provided insights into the diversity of diet in these early Neolithic communities, mainly based on terrestrial resources. We also present the first compound-specific radiocarbon dates for the region, obtained directly from absorbed organic residues extracted from pottery, identified as dairy lipids.

Vrbjanska Čuka is a tell site in the region of Pelagonia (Macedonia) established 8000 years ago by the Neolithic communities. Later it was used as an agricultural unit during the Roman era and the Middle Ages when it was also employed as a burial area. The excavations performed in the 1980s and during the last five years indicate a Neolithic farming society that constructed large buildings made of daub in a settlement enclosed by a circular ditch. The buildings had many clay structures, such as ovens, granaries, bins and grinding areas for processing cereals and bread production. The Neolithic communities used sophisticated fine pottery and modeled figurines and altars, while the stone tools were mainly used for cutting trees, harvesting and grinding. Apart from the cereal-based food (einkorn wheat, emmer wheat or barley), the inhabitants of Vrbjanska Čuka consumed lentils, peas and a variety of gathered wild fruits, while cattle, caprovine, mussels, fish and wild game meat was also part of a diet, as well as the dairy products. This paper will be a summary of a variety of data provided from the current international and multidisciplinary research of the site that involves excavation, prospection, geomagnetic survey, study of material culture, examination of architecture, radiocarbon dating, geoarchaeological, archaeobotanical, archaeozoological, lipid and use-wear analyses, as well as the topographic and 3D modeling. The recent knowledge on Vrbjanska Čuka provides novel understanding of the Early Neolithic in Pelagonia and contributes to the more extensive research of first farming societies in the Balkans.