There is a growing interest to immobilize desired bacteria using inexpensive materials in order to improve the wastewater treatment process. Three different types of carriers namely natural zeolite, magnesium-exchanged natural zeolite and quartz sand of different particle size were used to immobilize the phosphate-accumulating bacteria Acinetobacter calcoaceticus and to determine which one was the most effective. Bacteria were cultured for 24 h in various reactors containing different particle sizes of each of the carriers. The majority of the cultured bacterial population was immobilised onto the different carriers by means of adsorptive growth while a minority of free cells was observed in the supernatant. The number of immobilised viable cells (CFU) depended on the type of carrier and the particle size. The highest loading rate of immobilised cells (68.61±1.11 x 10 8 CFU/g) was observed with the smallest particle size (<0.125 mm) of magnesium-exchanged natural zeolite.
Samples of cretaceous limestone have been treated with three application methods (poultice, immersion and brushing) using different concentrations of ammonium oxalate solution (AmOx) and varying treatment time in order to test the efficiency of surface and in-depth formation of a protective layer of calcium oxalate (CaOx). Synchrotron-based microanalytical techniques (SR-µXRD with 12.5 µm × 7.5 µm (H × V ) probe size, SR-µFTIR with 10 µm × 10 µm and 8 µm × 20 µm probe sizes) and laboratory µFTIR, XRD and SEM have been employed for analysis of the treated samples. Synchrotron-based techniques showed variations in the CaOx distribution along the surface on a micrometer scale. All treatments resulted in the
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