Antiviral hydrophobic cellulose-based cotton or non-woven fabrics containing mesoporous TiO 2 particles were developed for potential use in healthcare and in other contaminated environments. Hydrosols made with the sol-gel method using two different amounts of the Ti precursor were applied to cotton and non-woven fabrics and their virucidal effect on Murine Coronavirus (MHV-3) and Human Adenovirus (HAdV-5) was evaluated under indoor light irradiation. The results show 90% reduction of HAdV-5 and up to 99% of MHV-3 in non-woven fabric, and 90% reduction of MHV-3 and no reduction of HAdV-5 in cotton fabric. The antiviral activity was related to the properties of the TiO 2 powders and coatings characterized by BET surface area, DRX, DLS, FTIR, DRS, SEM, TEM and water contact angle. The hydrophobic characteristic of the treated fabrics and the high surface area of the TiO 2 particles favor interaction with the virus, especially MHV-3. These results demonstrate that non-woven fabric and cotton, coated with TiO 2 , can be highly effective in preventing contamination with MHV-3 and HAdV-5 viruses, particularly for applications in healthcare indoor environments.