Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

The spectroscopic properties of galaxies in the Coma Cluster are compared with those of galaxies in rich clusters at z $ 0:5, to investigate the evolution of the star formation history in clusters. Luminous galaxies with M V À20 and poststarburst/post-star-forming (k+a) spectra that constitute a significant fraction of galaxies in distant cluster samples are absent in Coma, where spectacular cases of k+a spectra are found instead at M V > À18:5 and represent a significant proportion of the cluster dwarf galaxy population. A simple inspection of their positions on the sky indicates that this type of galaxy does not show a preferential location within the cluster, but the bluest and strongest lined group of k+a galaxies lie in projection toward the central 1.4 Mpc of Coma and have radial velocities significantly higher than the cluster mean. We find a striking correlation between the positions of these young and strong poststarburst galaxies and substructure in the hot intracluster medium (ICM) identified from XMM-Newton data, with these galaxies lying close to the edges of two infalling substructures. This result strongly suggests that the interaction with the dense ICM could be responsible for the quenching of the star formation (thus creating the k+a spectrum) and, possibly, for any previous starburst. The evolution with redshift of the luminosity distribution of k+a galaxies can be explained by a ''downsizing effect,'' with the maximum luminosity/mass of actively star-forming galaxies infalling onto clusters decreasing at lower redshift. We discuss the possible physical origin of this downsizing effect and the implications of our results for current scenarios of environmental effects on the star formation in galaxies.

Background and objectives: Cinacalcet, a novel calcimimetic, targets the calcium-sensing receptor to lower parathyroid hormone (PTH), calcium, and phosphorus levels in dialysis patients with secondary hyperparathyroidism (SHPT). This study compared the efficacy of a cinacalcet-based regimen with unrestricted conventional care (vitamin D and phosphate binders) for achieving the stringent National Kidney Foundation Kidney Disease Outcomes Quality Initiative (KDOQI) targets for dialysis patients.Study design: In this multicenter, open-label study, hemodialysis patients with poorly controlled SHPT were randomized to receive conventional care (n ‫؍‬ 184) or a cinacalcet-based regimen (n ‫؍‬ 368). Doses of cinacalcet, vitamin D sterols, and phosphate binders were adjusted during a 16-wk dose-optimization phase with the use of algorithms that allowed cinacalcet to be used with adjusted doses of vitamin D. The primary end point was the proportion of patients with mean intact PTH <300 pg/ml during a 7-wk efficacy assessment phase.Results: A higher proportion of patients receiving the cinacalcet-based regimen versus conventional care achieved the targets for PTH (71% versus 22%, respectively; P < 0.001), Ca ؋ P (77% versus 58%, respectively; P < 0.001), calcium (76% versus 33%, respectively; P < 0.001), phosphorus (63% versus 50%, respectively; P ‫؍‬ 0.002), and PTH and Ca ؋ P (59% versus 16%, respectively, P < 0.001), and allowed a 22% reduction in vitamin D dosage in patients receiving vitamin D at baseline. Achievement of targets was greatest in patients with less severe disease (intact PTH range, 300 to 500 pg/ml) and the cinacalcet dose required was lower in these patients (median ‫؍‬ 30 mg/d).Conclusions: Compared with conventional therapy, a cinacalcet-based treatment algorithm increased achievement of KDOQI treatment targets in dialysis patients in whom conventional therapy was no longer effective in controlling this disease.

The ages of stellar populations in 52 elliptical and S0 galaxies in the Coma cluster are investigated using a new spectroscopic sample. More than 40% of the S0s are found to have experienced recent star formation in their central regions during the last ∼ 5 Gyrs, while such activity is absent in the ellipticals. Galaxies in this sample have absolute magnitudes in the range −20.5 < M B < −17.5, and the fraction of S0 galaxies with recent star formation is higher at fainter luminosities. The observed luminosity range of S0 galaxies with signs of recent star formation activity is consistent with them being the descendants of typical star-forming spirals at intermediate redshift whose star formation has been halted as a consequence of the dense environment.