Supercritical fluid chromatography (SFC) has long been touted as the separation of choice for high throughput applications and, after nearly a decade, is finally considered mainstream in the analytical chemistry community. Applications range from microscale analysis of complex mixtures to macroscale purification of chiral enantiomers in a variety of industries, such as pharmaceuticals, foods, cosmetics, agrochemicals, petrochemical and natural products. The inherent speed, efficiency, and versatility of SFC have transformed the perceptions of the technology from novelty to integral tool for the modern analytical lab, especially those labs wanting to maximize throughput. However, maneuvering SFC through the transition from single samples to array-like libraries has strained the development of instrumentation to meet the challenging demands. This review presents the major developments and applications useful to those embarking on using SFC for high throughput applications in other fields.