Desktop analyzers that are commonly used in physicians' offices or at screening sites were discussed. Although some of these systems can achieve acceptable performance that meets LSP guidelines, some may not, especially in the hands of inexperienced or inadequately trained personnel.The second LSP report is in the final stage of preparation and is targeted for the manufacturer, the clinical laboratory, and those who use desktop analyzers. Detailed step-by-step guidelines and recommendations for standardizing cholesterol measurement are provided. National resources are described in sufficient detail to allow the user to access the resources, ensuring more reliable cholesterol measurement through the standardization program. This report outlines the technical and organizational elements necessary for overall reliability of cholesterol measurements. In addition, details are provided on preanalytic and analytic issues that affect cholesterol values, such as the biologic effects of age, gender, diurnal variation, long-term intraindividual variation, seasonal variation, and the effects of diet, alcohol, weight changes, exercise, and drugs. Also included are recommendations on posture, method of blood sampling, proper use of anticoagulants, and effects of myocardial infarction, stroke, cardiac catheterization, surgical trauma, and infection, factors known to influence cholesterol values. Details on the development of reference materials for calibration and quality control, issues concerning quality assurance, external surveillance programs, and desktop analyzers are provided.The workshop participants also discussed the clinical laboratory measurement of serum triglycerides and high density lipoprotein (HDL) cholesterol. It was noted that these assays are distinctly less accurate and precise than those for total cholesterol.

Background Acute uncomplicated cystitis (AUC) is a frequent infection that requires antibiotic treatment that is seen in women who seek care at urgent care centers.1 The Infectious Diseases Society of America (IDSA) has published guidelines for the management of AUC. They recommend nitrofurantoin, trimethoprim-sulfamethoxazole, or fosfomycin as the first-line agents for the treatment of AUC in healthy adult women.1 Previous evaluation of prescribing practices at Tower Health Urgent Care Centers demonstrated a 16% compliance rate with treatment guidelines for AUC. Subsequently, provider education and an AUC medication order set in the electronic medical record (EMR) were implemented. The purpose of this study was to evaluate the effectiveness of these interventions on prescribing practices for AUC. Methods A retrospective electronic chart review was performed evaluating the antibiotic treatment of patients diagnosed with AUC between January 1, 2018, to June 30, 2019, excluding July 2018 to September 2018, the washout period during which education was provided. Inclusion criteria included age ≥ 18 years, female, and treated at one of three Tower Health Urgent Care Centers. Results 1,455 patients met inclusion criteria during our study period. Our three-month washout period included 273 of those patients. 421 patients were included in the pre-education analysis with 761 patients in the post-education analysis. When both first and second-line antibiotic prescriptions were evaluated in conjunction with considering patient allergies and concomitant infections, compliance was 59% in our post-education analysis (improved from 16% compliance at baseline). Conclusion Provider education and an AUC medication order set in the EMR were successful in increasing the compliance rate with antibiotic treatment guidelines for the management of AUC in adult females in the urgent care setting. Disclosures All Authors: No reported disclosures

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