Over the past two decades, 292 Florida water reclamation facilities (WRFs) have collected reclaimed water protozoa data. This paperexamines the data set, which includes more than 1,800 observations of each protozoan, including Cryptosporidium and Giardia. This study considers the occurrence of protozoa across WRFs and the potential implications for potable reuse. Quantitative microbial risk assessment was conducted for reclaimed waters from 20 WRFs to assess the necessary log reduction credit to treat water from each facility for Cryptosporidium and Giardia. These results are compared with advanced treatment trains based on either reverse osmosis or ozone with biologically active filtration.