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In the last two decades, organizations have recognized, indeed fixated upon, the impOrtance of quality and quality management One manifestation of this is the emergence of the total quality management (TQM) movement, which has been proclaimed as the latest and optimal way of managing organizations. Likewise, in the domain of human resource management, the concept of quality of work life (QWL) has also received much attention of late from theoreticians, researchers, and practitioners. However, little has been done to build a bridge between these two increasingly important concepts, QWL and TQM. The purpose of this research is to empirically examine the relationship between quality of work life (the internalized attitudes employees' have about their jobs) and an indicatorofTQM, customer service attitudes, CSA (the externalized signals employees' send to customers about their jobs). In addition, this study examines how job involvement and organizational commitment mediate the relationship between QWL and CSA.
Although the liberal arts college, with its traditional focus on teaching, may seem like a natural environment for the scholarship of teaching and learning (SoTL), few such institutions participate in national SoTL initiatives. Our associates' experience since 2001 suggests a model for supporting SoTL in teaching‐intensive contexts based on faculty ownership, a focus on general education, and some emerging rules of engagement. Because faculty reward systems must validate SoTL if it is to become part of the institutional culture, we also describe one department's efforts to reform its review criteria in order to define scholarly activity broadly.
We describe a robust, fiducial-free method of drift correction for use in single molecule localization-based super-resolution methods. The method combines periodic 3D registration of the sample using brightfield images with a fast post-processing algorithm that corrects residual registration errors and drift between registration events. The method is robust to low numbers of collected localizations, requires no specialized hardware, and provides stability and drift correction for an indefinite time period.
Crosstalk between different receptor tyrosine kinases (RTKs) is thought to drive oncogenic signaling and allow therapeutic escape. EGFR and RON are two such RTKs from different subfamilies, which engage in crosstalk through unknown mechanisms. We combined high-resolution imaging with biochemical and mutational studies to ask how EGFR and RON communicate. EGF stimulation promotes EGFR-dependent phosphorylation of RON, but ligand stimulation of RON does not trigger EGFR phosphorylation – arguing that crosstalk is unidirectional. Nanoscale imaging reveals association of EGFR and RON in common plasma membrane microdomains. Two-color single particle tracking captured formation of complexes between RON and EGF-bound EGFR. Our results further show that RON is a substrate for EGFR kinase, and that transactivation of RON requires formation of a signaling competent EGFR dimer. These results support a role for direct EGFR/RON interactions in propagating crosstalk, such that EGF-stimulated EGFR phosphorylates RON to activate RON-directed signaling.
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