Objective of research was (a) to evaluate the influence of pollination-prevention on various metabolic parameters of the two maize inbreds B73 and B14A and their F1, and (b) to gain information on the inheritance of leaf senescence, in response to pollination-prevention. The results show that the visual pattern of leaf senescence, in response to prevention of ear pollination, contrasts markedly between the two inbred lines. Relative to control plants, prevention of ear pollination, causes a premature senescence in B73 and B73 x B14A plants, while leaves of unpollinated B14A remain green and similar in appearance to pollinated controls. Furthermore, prevention of ear pollination induces a sizable reduction of dry matter accumulation of all above-ground material and changes in various metabolic parameters. An accumulation of sucrose in the leaves of unpollinated B73 and B73 x B14A plants is correlated with the development of premature senescence. Finally, the genetic analysis supports suggestions that a single dominant gene is responsible for the differences observed, in the visual pattern of leaf senescence, in response to prevention of ear pollination.