Purpose To image retinal macrophages at the vitreoretinal interface in the living human retina using a clinical optical coherence tomography (OCT) device. Methods Eighteen healthy controls and three patients with retinopathies were imaged using a clinical spectral-domain OCT. In controls, 10 sequential scans were collected at three different locations: (1) ∼9 degrees temporal to the fovea, (2) the macula, and (3) the optic nerve head (ONH). Intervisit repeatability was evaluated by imaging the temporal retina twice on the same day and 3 days later. Only 10 scans at the temporal retina were obtained from each patient. A 3-µm OCT reflectance (OCT-R) slab located above the inner limiting membrane (ILM) surface was averaged. Results In controls, ramified macrophage-like cells with regular spatial separation were visualized in the temporal and ONH OCT-R images; however, cell structures were not resolvable at the macula. Interim changes in cell position suggestive of cell translocation were observed between images collected on the same day and those collected 3 days later. There was considerable variation in cell density and nearest-neighbor distance (NND) across controls. Mean ± SD cell densities measured at the temporal and ONH were 78 ± 23 cells/mm 2 and 57 ± 16 cells/mm 2 , respectively. Similarly, mean ± SD NNDs measured at the temporal and ONH were 74.3 ± 13.3 µm and 93.3 ± 20.0 µm, respectively. Nonuniform spatial distribution and altered morphology of the cells were identified in patients with retinopathies. Conclusions Our findings showed regular spatial separation and ramified morphology of macrophage-like cells on the ILM surface with cell translocation over time in controls. Their distribution and morphology suggest an origin of macrophage-like cells such as microglia or hyalocytes.

Vitreous cortex hyalocytes are resident macrophage cells that help maintain the transparency of the media, provide immunosurveillance, and respond to tissue injury and inflammation. In this study, we demonstrate the use of non-confocal quadrant-detection adaptive optics scanning light ophthalmoscopy (AOSLO) to non-invasively visualize the movement and morphological changes of the hyalocyte cell bodies and processes over 1-2 hour periods in the living human eye. The average velocity of the cells 0.52 ± 0.76 µm/min when sampled every 5 minutes and 0.23 ± 0.29 µm/min when sampled every 30 minutes, suggesting that the hyalocytes move in quick bursts. Understanding the behavior of these cells under normal physiological conditions may lead to their use as biomarkers or suitable targets for therapy in eye diseases such as diabetic retinopathy, preretinal fibrosis and glaucoma.

The effect of AlN/GaN superlattice (SL) buffer on the strain state in a GaN-on-Si(111) system was studied in detail by room-temperature micro-Raman scattering measurement. An abnormal satellite peak attached to a GaN E 2 peak was observed, which was verified to stem from the compressively strained GaN in SLs. The results indicate that the strain-sensitive GaN E 2 (high) peak in the GaN-on-Si system with AlN/GaN SLs splits into two peaks because the GaN stress state in the top GaN layer is different from that in SLs. The compressive stress in the GaN layer in SLs was introduced by the AlN layer in each SL period because of the lattice mismatch between GaN and AlN, which ultimately counterbalanced the tensile stress in the top GaN during cooling. Such a counterbalance interaction is strongly dependent on the stiffness coefficient of AlN/GaN SLs, which is proportional to the number of periods of SLs and the relative thickness of AlN in SLs. Such two E 2 peaks from GaN enable us to monitor the strain state in the GaN-on-Si system with AlN/GaN SLs quantitatively.

Both the forward and reverse-bias current transport mechanisms of an AlGaN/GaN Schottky barrier diode with a fully recessed Schottky anode (recessed-SBD) are investigated for the first time. A two-dimensional (2D) device simulation gives insight into the electronic transport. The difference between the forward and reverse conduction paths enables the reduction in Von without sacrificing the low reverse leakage current properties. The results of temperature-dependent current–voltage (T–I–V) measurements show that thermionic field emission (TFE) is the dominant current transport mechanism for forward bias. In the reverse-bias region above the pinch-off voltage, two mechanisms codetermine leakage currents, which contain Frenkel–Poole emission from the overlapped planar contact and tunneling from the recessed sidewall contact. Below the pinch-off voltage, the leakage currents are observed to have exponential temperature dependence, which may be consistent with trap-assisted tunneling (TAT).