Indonesia has an abundant production of fruits and vegetables that can satisfy the demands of domestic and export markets. One of the most serious problems in fruit exports is the damage and contamination caused by fruit fly attacks to horticultural produce. Taiwan government rejects 13 types of fruit and vegetable commodities from Indonesia due to infestation by several species of fruit fly, including Bactrocera papayae, B. zonata, and B. musae. The research was carried out in Turikale, Maros District, South Sulawesi, Indonesia. Various fruits and vegetables serving as hosts of fruit flies, including Capsicum annum (chilli), Solanum lycopersium (tomato), Avverhoa carambola (star fruit), Psidium guajava (guava), Artocarpus heterophylllus (jackfruit), are cultivated in the area. The study was initiated to identify the fruit fly species associated with vegetable and fruit crops in the study site. Fruits and vegetables attacked by fruit flies were collected from the field and then separately kept in plastic containers in the laboratory until the adults emerged. The emerging flies were identified under a dissecting microscope based on the identification keys. Five species of fruit flies belonging to the Genus Bactorcera emerged from the collected fruits and vegetables, namely: