Differentiation between organic and conventional coffee has increased due to the growing demand and high consumption of healthy foods that contain compounds with antioxidant potential, which have been associated with the reduction of chronic diseases. We used organic and conventional coffee in powder 4% (w/w) and infusions 5%, 10% and 20% (w/v) incorporated in a commercial diet to test in vivo. The levels of chlorogenic acid, caffeine and trigonelline were determined by high performance liquid chromatography. The body weight, weight gain, food consumption, aberrant foci crypt, mucin depleted foci, stress biomarkers protein carbonyl and malondialdehyde, biochemical parameters and behavior of the rats were compared between the experimental and control groups within a framework of colon carcinogenesis. The organic coffee showed higher levels of chlorogenic acid, caffeine and trigonelline than conventional, however, this difference did not significantly affect behavior. The infusions had an antioxidant effect, reducing the levels of malondialdehyde; however, the biochemical parameters of the serum were not altered, and there was neither induction nor prevention of preneoplasic lesions.

This study aimed to evaluate the effect of organic/conventional coffee in liver tissues in the cancer process, taking into account the level and activities of catalase. The experiments were carried out with 8 groups of rats during 12 weeks. They received two injections of ethylenediaminetetraacetic acid solution 1.5% (v/v) prepared in 0.9% NaCl or 1,2-dimethylhydrazine (DMH) subcutaneous dose of 40 mg·kg−1·bw−1for 2 weeks. The organic/conventional coffee infusions were at 5, 10, and 20% and were incorporated to feed (100 mL of infusion·kg−1of diet). The catalase activity showed a decrease for livers which received DMH and DMH plus organic coffee at 5% and 10%. However, an increase was observed for those receiving organic 20% and conventional 10% coffee, slowing down and favoring the reversibility of the carcinogenic process. By SDS-PAGE, we observed an intensity decrease of 59 kDa bands, as the percentage of coffee was increased. The iron concentration (by ET-AAS) confirmed the electrophoretic results, suggesting that the DMH influenced the catalase expression conditions, reducing the activity by the loss of iron ions. Thus, the coffee may restore the catalase system in the liver, exerting its chemopreventive effects.

2016-12-23T18:47:23

Resumo: Dos problemas mais graves relacionados à poluição ambiental é a contaminação por metais pesados. Os métodos convencionais para remover metais de soluções aquosas incluem; a precipitação química, a troca iônica e processos de separação com membranas. No entanto, a aplicação de tais processos é, em alguns casos, inadequada devido a aspectos técnicos e econômicos. Uma boa proposta de tratamento para esses resíduos seria o processo de biossorção. Biossorção é uma propriedade que certos tipos de materiais inativos de origem biológica possuem, para captar e acumular metais pesados de soluções muito diluídas. O presente trabalho objetivou avaliar a potencialidade das cascas de maracujá quimicamente modificadas como biossorventes no tratamento de soluções aquosas contendo Chumbo. As biomassas foram modificadas com HCl a 1 mol.L -1 ou NaOH a 1 mol.L -1 . Logo após determinados ponto de carga zero e estudos de adsorção em função da variação do pH. Os resultados demostraram que a superfície das biomassas apresentaram ponto de carga zero entre as faixas 7 e 8. As biomassas estudadas possuem uma boa capacidade de biossorção, removendo até 73,6% de metal e com valor de pH na faixa de 9, As biomassas tratadas com base (HCl) obtiveram melhores resultados de remoção de Pb. Assim, o material mostra-se eficiente na remoção dos metais empregados.Palavras-chave: Poluição ambiental; Metais pesados; Biomassa.

No abstract