Context and motivation]Ensuring privacy of users' data has become a top concern in software development, either to satisfy users' needs or to comply with privacy laws. The problem may increase by the time a new law is in the vacancy period, and companies are working to understand how to comply with it. In addition, research has shown that many developers do not have sufficient knowledge about how to develop privacy-sensitive software. [Question/problem] Motivated by this scenario, this research investigates the personal factors affecting the developers' understanding of privacy requirements during the vacancy period of a data protection law. [Principal ideas/results] We conducted thirteen interviews in six different private companies. As a result, we found nine personal factors affecting how software developers perceive and interpret privacy requirements. [Contribution] The identification of the personal factors contributes to the elaboration of effective methods for promoting proper privacy-sensitive software development.

Introduction Immobilisation of biomaterials has been widely used in many fields, such as industry, biochemistry and immunology (Cheetham, 1985; Hermanson et al., 1992). This technology employs a large number of natural and artificial materials as matrices (Kennedy and Cabral, 1987). In our laboratory, we have proposed immobilisation procedures using dacron (Carneiro Leão et al., 1994), polyaniline (Nadruz et al., 1996) and glyptal (Jordão et al., 1996). Here, polyvinyl alcoholglutaraldehyde (PVA-glutaraldehyde) network is described for antigen and enzyme immobilisation. Previously, this material was used for the diagnosis of plague as a solid-phase in ELISA (Araujo et al., 1996) and also in laser induced fluorescence (Carvalho et al., 1996). Materials and methods Synthesis of PVA-glutaraldehyde beads Polyvinyl alcohol (2.0 g; Reagen ®) was dissolved in deionised water (20 mL) containing sodium dodecylsulfate (10 mg; Sigma) under heating and stirring. Glutaraldehyde (25%, w/v; 4.12 mL; Sigma) and H 2 SO 4 (0.3 M; 1 mL) were successively added to the dissolved PVA and then the solution was slowly poured into a beaker containing mineral oil/water mixture (45:45; v/v) with stirring. After about 15 min, PVA-glutaraldehyde beads were synthesised. In order to remove oil and sodium dodecylsulfate the beads were exhaustively washed with ethanol 95% v/v (500 mL) and deionised water (1 L). Synthesis of PVA-glutaraldehyde discs According to Araujo et al. (1996). Enzyme immobilisation ␣-Amylase (20 mL of 65 U/mg protein; Termamyl, Novo Nordisk, Paraná, Brazil) prepared in 50 mM citrate-phosphate buffer, pH 4.5, was incubated with PVA-glutaraldehyde beads (1 g) under stirring overnight at 4°C. After immobilisation, the water insoluble enzymatic derivative was washed with 1 M NaCl (100 mL) in buffer. Amyloglucosidase (20 mL of 6.5 U/mg protein; AMG, Novo Nordisk, Paraná, Brazil), prepared in 50 mM citrate-phosphate buffer, pH 6.5, was incubated with PVA-glutaraldehyde as described above. Xanthine oxidase (10 mL; Nutritional Biochemichals Corporation) prepared in 0.1 M phosphate buffer, pH 8.0, was incubated with PVAglutaraldehyde beads (0.6 g) under mild stirring overnight at 4°C. Then, the immobilised enzymatic derivative was exhaustively washed with deionised water and 1 M NaCl, successively. Afterwards, overnight incubation with 1 M glycine was carried out to inactivate free carbonyl groups and washings were carried out again.

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