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The bioemulsifier secreted by strain XS2, which was isolated from oil-contaminated soil samples in Yumen oil field, China, was partially purified and analyzed. Protease hydrolysis and heat treatment results showed that the protein composition of the bioemulsifier played a significant role in emulsification activity. High-performance liquid chromatography (HPLC) study suggested that the protein composition may exist as glycoprotein. Monosaccharide analysis by gas chromatograph-mass spectrometer (GC-MS) showed that galactose, glucose, xylose, and mannose presented in the bioemulsifier. The emulsification activity of this bioemulsifier was relatively high due to different carbohydrates (n-hexadecane 61 %, liquid paraffin 60 %, Toluene 77 %). Furthermore, single well stimulation trial in Changqing oil field, was done to explore the potential application in Microbial-enhanced oil recovery (MEOR). Up to September 2014, the cumulative increased crude oil production was about 88 T in total 240 days, and the water content of the well-produced liquid decreased from 85 to 25 %. These data illustrated that the bioemulsifier performed a high potential in applications and had important economic values.Keyword Bioemulsifier Á XS2 Á Chemical characterization Á MEOR Á Single well stimulation D. Dong and X. Wang are contributed equally to this work.

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