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Synthesis of Ag-doped magnetic composites with antibacterial properties is in the great interest in the development of new biomedical applications. Ag/γ-Fe2O3 composite microspheres have been successfully prepared via a simple solvothermal reduction method under mild conditions. The crystal phases and the shapes of the products were characterized by XRD and SEM. The results revealed that the composites are spherical with the diameter of 200-300 nm. Magnetic measurements showed that the mixed microspheres exhibit a typical ferromagnetic behavior, a specific saturation magnetization of 56 emu/g and an intrinsic coercivity of 38 Oe at room temperature. The strain of E. coli was used to evaluate the antibacterial activity of the Ag-doped γ-Fe2O3 composites which exhibited very high inhibition.