The use of vented clamshells has become popular in the packaging of grapes for local and international markets. The aim of this study is to evaluate the postharvest preservation of ‘Benitaka’ table grapes individually packaged in vented clamshells using different types of SO2-generating pads and perforated plastic liners during cold storage. A completely randomized design with four replications in a two-factor arrangement with an additional treatment [(4 × 3) + 1] was used. The trials were carried out under two situations: Artificial or natural infections with Botrytis cinerea, which is the causal agent of gray mold on table grapes. The incidence of gray mold, shattered berries, and stem browning were evaluated at 30 and 45 days of cold storage at 1 ± 1 °C and 3 days of shelf-life at 22 ± 1 °C after the period of cold storage. Mass loss and berry firmness were also examined at the end of the cold storage period. The use of dual-release SO2-generating pads containing 5 or 8 g of a.i. and slow-release pads with 7 g of a.i. was effective in controlling the incidence of gray mold in grapes packaged in vented clamshells and kept under cold storage for up to 45 days. Under these storage conditions, perforated plastic liners with 0.3% ventilation area or micro-perforated liners with 1.0% ventilation area reduced the percentage of mass loss and shattered berries.

The purpose of this research is to test the efficacy of different types of SO2-generating pads on the incidence of gray mold, and on the physicochemical properties of quality of ‘BRS Nubia’ seeded table grapes grown under protected cultivation. Four types of SO2-generating pads, 5 or 8 g of sodium metabisulfite dual release pads, and 4 or 7 g of sodium metabisulfite slow release pads, were used. Grapes bunches were harvested from a vineyard covered with plastic mash and stored in a cold room at 1 ± 1 °C for 45 days followed by 6 days of shelf life at 22 ± 1 °C at a high relative humidity (>95%). The results showed that SO2-generating pads with a dual release of 5 or 8 g completely inhibited the development of gray mold at all evaluation times. Also, a high reduction of the disease incidence was achieved by using a slow release of 4 g. The study confirmed that SO2-generating pads did not alter the physicochemical properties of ‘BRS Nubia’ seeded table grapes including mass loss, berry firmness, color index, total anthocyanin concentration, total soluble solids (TSS), titratable acidity (TA), and the TSS/TA ratio. Slow release pads at 4 and 7 g reduced the percentage of shattered berries by 56 and 48% as compared to control only after 6 days of shelf life. Also, all types of SO2-generating pads reduced the stem browning score at the end of cold storage. The 5 or 8 g dual release pads and 4 g slow release pads can be considered for effective controlling of gray mold for ‘BRS Nubia’ table grapes grown under protected cultivation while maintaining grape quality.

The aim of this work was to evaluate different SO2-generating pads and liners to control gray mold in ventilated clamshell-packaged ‘Rubi’ table grapes grown under a two-cropping per year system. The treatments consisted of SO2-generating pads (slow release or dual release) and plastic liners with different perforations (microperforated; 2.0; 4.0 or 5.0 mm in diameter) and a control, only with the standard microperforated plastic liner. The packaged grapes were stored in a cold chamber at 1.0 ± 1.0 °C and 95% relative humidity. After 45 days, the grapes were removed from cold storage and placed, without liners and SO2-generating pads, for 3 days at room temperature (22.0 ± 1.0 °C). The evaluations occurred at 30 and 45 days after the beginning of cold storage, and the following variables were assessed: incidence of gray mold, mass loss, stem browning and shattered berries. At 3 days of shelf-life, the same variables were assessed, except mass loss. The completely randomized design was used as a statistical model with four replications, and each plot consisted of five bunches individually stored in ventilated clamshell-packaged. The dual release SO2-generating pads are efficient in controlling the gray mold in ‘Rubi’ table grapes regardless of the type of perforation of the plastic liners, with low mass loss and shattered berries, with good conservation of the freshness of the rachis. The disease was efficiently controlled in both annual crops. The slow-release SO2-generating pads, regardless of the type of perforation of the plastic liners, resulted in intermediate efficiency of gray mold control, with good physical quality of the bunches. Thus, the use of dual release SO2-generating pads is recommended to control gray mold in ventilated clamshell-packaged ‘Rubi’ table grapes.

The objective of this work was to evaluate the rooting of herbaceous cuttings of CAMB-06 and APU-04 acerola cherry selections associated with the use of indolebutyric acid (IBA) and a concentration of aqueous extract of coco-grass. Cuttings were treated by immersion with different concentrations of IBA (0; 1,000; 2,000; 3,000; 4,000 mg L -1 ) and one application of aqueous extract of coco-grass in proportion 100% and placed to root in plastic boxes containing vermiculite. The experimental design was a completely randomized in a 2×6 factorial arrangement (two acerola cherry selections and six solutions for rooting) with four replicates of ten cuttings. After 90 days of cutting, the following variables were evaluated: cutting survival (%); leaf retention (%); rooted cuttings (%); sprouting (%); number of roots per cutting (%); length of roots (cm) and dry mass of roots per cutting (g). It was concluded that the application of IBA at 4,000 mg L -1 presented a better root development, resulting in a higher percentage of rooted cuttings and higher number and dry mass of roots in relation to IBA at 0 mg L -1 for CAMB-06 selection, while the APU-04 selection can be efficiently multiplied by herbaceous cuttings without the use of IBA. Under the conditions that the trials was conducted, the application of 100% aqueous extract of coco-grass was not effective in promoting the rooting of cuttings of CAMB-06 and APU-04 acerola cherry selections. Enraizamento de estacas deMalpighia emarginata D.C. (seleções CAMB-06 e APU-04) associadas ao uso do ácido indolbutírico e extrato aquoso de Cyperus rotundus L.Resumo -O objetivo deste trabalho foi avaliar o enraizamento de estacas herbáceas das seleções de acerolas CAMB-06 e APU-04 associadas ao uso de ácido indolbutírico (AIB) e uma concentração de extrato aquoso de tiririca. As estacas foram tratadas por imersão com diferentes concentrações de AIB (0; 1.000; 2.000; 3.000 e 4.000 mg L -1 ) e uma aplicação de extrato aquoso de tiririca, na proporção 100%, e dispostas para enraizar em caixas plásticas contendo vermiculita. O delineamento experimental foi inteiramente casualizado, em arranjo fatorial 2×6 (duas seleções e seis soluções para enraizamento) com quatro repetições de dez estacas. Após 90 dias da estaquia, foram avaliadas: sobrevivência das estacas (%); retenção foliar (%); estacas enraizadas (%); brotação (%); número de raízes por estaca (%); comprimento de raízes (cm) e massa seca de raízes por estaca (g). Concluiu-se que a aplicação de AIB na concentração 4.000 mg L -1 apresentou melhor desenvolvimento radicial, resultando em maior porcentagem de estacas enraizadas e maior número e massa seca de raízes em relação à concentração 0 mg L -1 de AIB para a seleção CAMB-06, enquanto a seleção APU-04 pode ser propagada por estaquia herbácea com eficiência, sem o uso de AIB. Nas condições em que o experimento foi conduzido, a aplicação de 100% de extrato aquoso de tiririca não foi efetiva na promoção do enraizamento de estacas das seleções de acerola CAMB-06 e APU-04. Termos para ...

This work aimed at evaluating the post-harvest conservation of “Benitaka” table grape by using different types of SO2-generating pads and perforated plastic liners under cold storage. The grapes were harvested from off-season crop 2018 in a commercial vineyard located at Cambira, PR, Brazil. The completely randomized design was used with four replications in a 2-factor arrangement with an additional treatment [(4 × 3) + 1], and the following factors were evaluated: a) SO2-generating pads (slow release containing 7 g of active ingredient – a.i.; slow release containing 4 g of a.i.; dual release containing 5 g of a.i.; and dual release containing 8 g of a.i.); and b) perforated plastic liners (0.3%, 0.9% and 1.0% of ventilation areas). The additional treatment did not contain any type of SO2-generating pad, only standard microperforated plastic liner (1% of ventilation area). The bunches were packed in 0.5 kg-capacity plastic clamshells, which were placed in a corrugated carton box with the different combinations of SO2-generating pads and plastic liners under cold storage at 1 ∘C with high relative humidity (>90%). The treatments were evaluated at 30 and 45 days after the beginning of the cold storage, recording the incidence of gray mold, shattered berries, stem browning, mass loss and berry firmness. The data were submitted to analysis of variance and the means were compared by Tukey's test at 5%. The use of dual release SO2-generating pads containing 5 g or 8 g of a.i., as well the slow release pads with 7 g of a.i., are efficient to control the incidence of gray mold in “Benitaka” table grapes packaged in plastic clamshells and kept under cold storage at 1 ∘C up to 45 days. In these storage conditions, the use of perforated plastic liners with 0.3% of ventilation area or the micro perforated with 1.0% of ventilation area, result in the lowest bunch mass loss and shattered berries.