plants are reported to be good sources of health-beneficial compounds such as fatty acid, which can generate livelihood and address food and nutritional insecurity 9) .Among others, Prinsepia utilis belongs to the family Rosaceae and is commonly known as Himalayan Cherry (English) ; Bhekhal, Jatalu, and Dhatila (Hindi) is one of the WEP found in the Himalayan region. It is a deciduous shrub found between 1000-3000 m asl and used in Chinese & Indian medicine to cure skin diseases, rheumatism, inflammation, and leprosy 10) . The seeds of the species are a source of edible oil and are reported as good as Mediterranean olive oil 11) . The seeds are rich in palmitic, oleic, linoleic, and erucic acid 9) . Besides cooking, the oil is used in arthritis, body pain, and muscular pain 11) . The seed oil is used in cosmeceuticals, skincare formulations and works as a skin barrier due to the presence of unsaturated fatty acids and vitamins 12) . The whole plant is traditionally used in many aliments such as infantile eczema, myogenic toxic-Abstract: The study aims to identify the fatty acid composition in Prinsepia utilis seeds collected from diverse populations of Western Himalaya, India. The seeds contained higher saturated fatty acid (SFA-43.09%; Average of all populations) followed by polyunsaturated fatty acid (PUFA -29.57%) and monounsaturated fatty acid (MUFA -25.53%), however variations among growing populations was measured. The correlation analysis reveals a significant (p < 0.05) negative connection between 10-Octadecenoic acid methyl ester (FA1), and altitude. Edaphic factors like available nitrogen showed significantly (p < 0.05) but the negative correlation with cis-11-Eicosenoic acid, methyl ester (FA10; p < 0.001), Docosanoic acid, methyl ester (FA11; p < 0.05), Heptadecanoic acid, methyl ester (FA14; p < 0.05), and Tricosanoic acid, methyl ester (FA29; p < 0.05). Antioxidant activity did not show any variation in the seed oil of different populations but varied among the concentrations (5-50 µL) used. IC 50 values of DPPH activity was significantly (p < 0.05) varied connect ranged from 11.31-24.21 µL/mL among the different populations. These variations in fatty acid composition and IC 50 values of the DPPH activity can be one of the indicators for selecting a promising population that would be harnessed for its potential in nutraceuticals and cosmeceuticals industries.