Biofouling negatively impacts water treatment performance of membranes by reducing water permeability, increasing energy consumption, and shortening the lifetime of the membranes. In this study, we integrated the bactericidal property of silver nanoparticles (AgNPs) with hydrophilicity of hydrogels to modify membranes that not only reduce adhesion, but also deactivate the adhered bacteria. Two approaches for AgNP synthesis were adoptedin situ synthesis and encapsulation in single step, and immobilization in multistep. Formation of AgNPs was confirmed by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD) studies. Compared to the pristine membrane, AgNP/hydrogel-modified membranes displayed no adverse effect in water flux under gravitational flow condition. The AgNP/hydrogel-modified membranes also exhibited better antibacterial properties (inhibition of adhesion and growth of Escherichia coli) as demonstrated by the bacterial growth, inhibition zone, and coculture (with the membranes) studies. The improvements could be attributed to the synergistic effect of hydrophilic hydrogel networks and the presence of bactericidal AgNPs. In addition, comparison of the antibacterial studies revealed the superiority of the encapsulated AgNPs over the immobilized AgNPs. This could be attributed to the efficient release of the former over the latter. To the best of our knowledge, this is the first study that demonstrates the enhancement of antibacterial properties of membrane via in situ synthesis and encapsulation of AgNPs within hydrogel matrices.