The objective of this prospective follow-up trial was to ascertain whether the urinary kidney injury molecule-1 (uKIM-1) associates with tumor tissue (tKIM-1) expression and with the pathological characteristics of clear renal cell carcinoma (cRCC) in radically nephrectomized (RN) and/or in partially nephrectomized (PN) patients with cRCC, pre- and postoperatively. This clinical study included 40 patients subjected to RN/PN (cRCC group) and 30 healthy volunteers (control group). Urinary KIM-1 was determined by ELISA TIM-1/KIM-1 kit and normalized by urinary creatinine. Immunohistochemical staining (monoclonal anti-human anti-TIM-1/KIM-1/HAVCR antibody) was used for semiquantitative analysis of the tKIM-1 expression and expressed as a score (% KIM-1 positively stained tubules). Both markers were interpreted in terms of the tumor characteristics comprising tumor size, Fuhrman grade, pathological (pT) stage, tumor/nodes/metastasis (TNM) stage, lymphovascular invasion and type of surgery RN/PN. Preoperative uKIM-1 was significantly higher in the cRCC group compared to controls, such as uKIM-1 was statistically higher in RN than in PN patients. Postoperatively, uKIM-1 decreased to control values. Expression of tKIM-1 was documented in all nephrectomized patients. Significant associations were achieved between uKIM-1 and tKIM-1 and with considered tumor characteristics, especially with tumor size and grade. Based on the accomplished associations, we found uKIM-1 as a highly sensitive marker for cRCC diagnosis. The clinical trial registration number: 1110-2012.

Introduction Aortic valve calcification (AVC) accelerates development of aortic valve stenosis and cardiovascular complications. Hyperphosphatemia is one of the key risk factors for aortic valve calcification. Aim The aim of this study was to evaluate the prevalence of AVC in patients on regular hemodialysis and to assess the impact of different factors on its appearance. Method: The study investigated a total of 115 patients treated in the Hemodialysis Department of the Urology and Nephrology Clinic at the Kragujevac Clinical Center in Serbia. The variables investigated were: serum albumin, C-reactive protein (CRP), homocysteine, total cholesterol, LDL-cholesterol (LDL-C), HDL-cholesterol (HDL-C), triglycerides (TG), Apolipoprotein A-I (Apo A-I), Apolipoprotein B (Apo B) and lipoprotein (a), calcium, phosphate and parathormone, and calcium-phosphorus product (Ca × P). Patients were evaluated by echocardiography for AVC. Statistical analysis included univariate and multivariate logistic regression analysis. Results Univariate regression analysis showed that serum phosphate levels and Ca × P are the most important risk factors for AVC (p<0.001). Multivariate logistic regression analysis revealed that hyperphosphatemia is an independent risk factor for AVC (p<0.001). Conclusion Hyperphosphatemia is an independent risk factor for aortic valve calcification.

Patients on HD have high risk for cardiovascular morbidity and mortality.

Cardiovascular diseases represent the main cause of death in hemodialysis (HD) patients. Cardiac troponins (cTnT and cTnI) are indicators of myocardial damage. The aims of this study were to assess the prevalence of increased serum cTn in the absence of acute coronary syndrome, to determine overall and cardiovascular mortality rates, and to investigate the possible predictive values of cTnT and cTnI on the outcome in HD patients over a 2-year follow-up period. The study included 115 patients (71 men and 44 women) with an average age of 53.30 +/- 12.17 years who had undergone regular HD for 4.51 +/- 4.01 years and had a mean HD adequacy (Kt/Vsp) of 1.17 +/- 0.23. Increased serum cTnT concentration was found in 37.39% of patients and elevated serum cTnI concentration was present in 11.30% of HD patients without symptoms or signs of acute coronary syndrome. The average 2-year mortality rate was 13.74% and the average 2-year cardiovascular mortality rate was 8.51%. Patients with serum cTnT levels greater than 0.10 ng/ml had significantly lower overall and cardiovascular survival rates than patients with serum cTnT levels of less than 0.10 ng/ml. Patients with serum cTnI levels greater than 0.15 ng/ml had significantly lower overall and cardiovascular survival rates than patients with serum cTnI of less than 0.15 ng/ml. In patients on regular HD, cTn levels are significant outcome predictors.