Background:The bicipital groove (BG) or intertubercular sulcus lies between the greater and lesser tubercles on the proximal end of humerus. This groove acts as a useful landmark for proper placement of lateral fin of a prosthesis in shoulder replacement surgery. The variations of BG may lead to various pathologies of biceps tendon which is one of the common causes of shoulder pain. Hence this study is undertaken to examine the various morphometric parameters of BG in South Indian population. Materials and Methods:The study was conducted on 98 dry human humeri (48 right side and 50 left side) belonging to south Indian race. The length, depth and width of BG were measured with digital vernier calliper. The opening and medial wall angles of BG were measured with help of scale and goniometer. Results:The mean length of medial wall, lateral wall, width and depth of BG were 23 ± 4 mm, 31 ± 4 mm, 8.4 ± 01 mm and 5 ± 1 mm respectively. The average medial wall angle and opening angles of BG were 48.72 ± 9.37° and 82.9 ± 20.5° respectively. The supratubercular ridge of Meyer was observed in 26.5 % of humerus. Conclusion:We hope that this study will be an important reference for anthropologists, clinical anatomists and for scientific research.